Immunochromatographic assay as diagnostic tool for neglected diseases: studies of improvement, scheduling and feasibility

• Grant number: 13/06589-6
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2013
• End date: December 31, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Roxane Maria Fontes Piazza
• Grantee: Roxane Maria Fontes Piazza
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Luis Carlos de Souza Ferreira

Abstract

Immunochromatographic tests (IC) have been widely applied to different pathogens, to permit quick results, low cost, long-term stability, easy application and interpretation of the results. Considering these properties, the test IC can be employed in places of low income and few laboratory resources, predominantly in the detection of diseases of limited interest to industrialized countries, essentially neglected diseases such as diarrhea and dengue. The diarrheal disease is considered a major public health worldwide problem causing about 1.5 million annual deaths in children under five years old. Among the pathogens, the diarrheagenic Escherichia coli are responsible for 30-40% of episodes of acute diarrhea in developing countries. The application of specific antibodies in the development of an immunoassay (IC) identified ETEC, STEC and EPEC through their toxins and secreted proteins, allowing their future use in clinical laboratories and hospitals. Dengue in Brazil is considered an epidemic disease, thus the rapid diagnosis can short-term an adequate medical care and thus help to contain the spread of the disease. As noted, the diagnosis is an essential tool to minimize and control occurrences of these diseases. Thus, the purpose of this project is to develop and improve diagnostic tests immunochromatographic rapid and low cost for diarrhea and dengue. Moreover, design scaling studies to the standardized tests, validating the feasibility of larger scale production. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAETANO, BRUNA ALVES; ROCHA, LETICIA BARBOZA; CARVALHO, ENEAS; FONTES PIAZZA, ROXANE MARIA; LUZ, DANIELA. Immunogenic Domains and Secondary Structure of Escherichia coli Recombinant Secreted Protein Escherichia coli-Secreted Protein B. FRONTIERS IN IMMUNOLOGY, v. 8, APR 24 2017. (11/12928-2, 13/06589-6)

ROCHA, LETICIA BARBOZA; DOS SANTOS ALVES, RUBENS PRINCE; CAETANO, BRUNA ALVES; PEREIRA, LENNON RAMOS; MITSUNARI, THAIS; AMORIM, JAIME HENRIQUE; POLATTO, JULIANA MOUTINHO; BOTOSSO, VIVIANE FONGARO; FRAZATTI GALLINA, NEUZA MARIA; PALACIOS, RICARDO; et al. Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies. ANTIBODIES, v. 6, n. 4, DEC 2017. (13/06589-6, 14/17595-0, 12/09096-8)

KANAMURA, CRISTINA TAKAMI; FONTES PIAZZA, ROXANE MARIA; IGLEZIAS, SILVIA D. 'ANDRETTA; BORGES, CINTHYA CIRQUEIRA; SANSONE, MARCELO; POLATTO, JULIANA MOUTINHO; TEIXEIRA, DAYANE LIMA; DA SILVA, MIRIAM APARECIDA; ROCHA, LETICIA BARBOZA; NOGUEIRA, JULIANA SILVA; et al. Anti-DENV-NS1 monoclonal antibody for the differential histopathological diagnosis of hemorrhagic fever caused by dengue. Brazilian Journal of Microbiology, v. 53, n. 2, p. 7-pg., 2022-02-07. (13/06589-6)

SILVA, MIRIAM A.; SANTOS, ANNA RAQUEL R.; ROCHA, LETICIA B.; CAETANO, BRUNA A.; MITSUNARI, THAIS; SANTOS, LUANDA I.; POLATTO, JULIANA M.; HORTON, DENISE S. P. Q.; GUTH, BEATRIZ E. C.; DOS SANTOS, LUIS FERNANDO; et al. Development and Validation of Shiga Toxin-Producing Escherichia coli Immunodiagnostic Assay. MICROORGANISMS, v. 7, n. 9, SEP 2019. (12/02618-9, 13/06589-6)

HENRIQUE, CAMILA; CAETANO, BRUNA A.; MITSUNARI, THAIS; DOS SANTOS, LUIS FERNANDO; PIAZZA, ROXANE M. F.; ROCHA, LETICIA B.. Large-scale evaluation of a rapid diagnostic test for diarrhea caused by enterotoxigenic Escherichia coli targeting the heat-labile toxin. Journal of Microbiological Methods, v. 144, p. 125-127, JAN 2018. (13/06589-6)

GIOIA-DI CHIACCHIO, ROSELY MARTINS; VIEIRA CUNHA, MARCOS PAULO; MARQUES DE SA, LILIAN ROSE; DAVIES, YAME MINIEIRO; PACHECO PEREIRA, CAMILA BUENO; MARTINS, FERNANDO HENRIQUE; MUNHOZS, DANIELLE DIAS; ABE, CECILIA MARI; FRANZOLIN, MARCIA REGINA; DOS SANTOS, LUIS FERNANDO; et al. Novel Hybrid of Typical Enteropathogenic Escherichia coli and Shiga-Toxin-Producing E-coli (tEPEC/STEC) Emerging From Pet Birds. FRONTIERS IN MICROBIOLOGY, v. 9, DEC 6 2018. (13/06589-6, 16/12641-9)

MUNHOZ, DANIELLE D.; NARA, JULIA M.; FREITAS, NATALIA C.; MORAES, CLAUDIA T. P.; NUNES, KAMILA O.; YAMAMOTO, BRUNO B.; VASCONCELLOS, FRANCIELLI M.; MARTINEZ-LAGUNA, YGNACIO; GIRON, JORGE A.; MARTINS, FERNANDO H.; et al. Distribution of Major Pilin Subunit Genes Among Atypical Enteropathogenic Escherichia coli and Influence of Growth Media on Expression of the ecp Operon. FRONTIERS IN MICROBIOLOGY, v. 9, MAY 15 2018. (13/06589-6, 09/14845-7, 04/12136-5)