Plant uncoupling mitochondrial proteins: functional analysis employing RNA-seq and knockout mutants

• Grant number: 13/12947-2
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2013
• End date: March 31, 2016
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Ivan de Godoy Maia
• Grantee: Ivan de Godoy Maia
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant(s): 15/50014-3 - The auto-regulatory loop involving miR828, TAS4, and target MYB transcription factors: basic and applied studies, AP.R SPRINT

Abstract

The main objective of this project is to determine the whole gene expression profiles of transgenic tobacco plants overexpressing an uncoupling mitochondrial protein from Arabidopsis thaliana (AtUCP1) using RNA-Seq. Differences in the transcriptome profiles between untransformed and derived AtUCP1 transgenic lines will be evaluated. In parallel, knockout mutants for each AtUCP gene present in the Arabidopsis genome will be obtained and functionally characterized. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NUNES LAITZ, ALESSANDRA VASCONCELLOS; ACENCIO, MARCIO LUIS; BUDZINSKI, ILARA G. F.; LABATE, MONICA T. V.; LEMKE, NEY; MARTINS RIBOLLA, PAULO EDUARDO; MAIA, IVAN G.. Transcriptome Response Signatures Associated with the Overexpression of a Mitochondrial Uncoupling Protein (AtUCP1) in Tobacco. PLoS One, v. 10, n. 6, JUN 24 2015. (13/12947-2)