Histopathological, biochemical and molecular analyzes on the effect of physical exercise and high doses of nandrolone Deconoate during the post-pubertal on aging rat prostates

• Grant number: 13/07203-4
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2013
• End date: February 28, 2015
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Raquel Fantin Domeniconi
• Grantee: Raquel Fantin Domeniconi
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Patricia Fernanda Felipe Pinheiro ; Wagner José Fávaro ; Wellerson Rodrigo Scarano

Abstract

Anabolic-androgenic steroids are synthetic drugs derived from testosterone which stimulate the protein synthesis (anabolic effect). For this reason, they are used by professional athletes, recreational athletes, prepubescents and adolescents who want to increase muscle mass, strength and performance in physical activities. High doses of anabolic-androgenic steroids may be an etiological factor in male infertility, prostate cancer and coronary heart disease. The Nandrolone Decanoate (ND) is the most widely used steroid among athletes. The aim of this study is to investigate the combined effects of exercise and high doses of ND on the prostate structure and morphophysiology of postpubertal and aging rats. It is intended to evaluate whether ND, with or without exercise in post-pubescent, interferes in the aging prostate morphophysiology. Eight groups (07 animals each) of male Sprague-Dawley rats, 90 days old, will be treated for 8 weeks. The animals will be grouped according to the treatment: sedentary or exercising animals with or without DN. The jumping into water protocol will be performed for 3 days per week. Four animal groups will be euthanized in CO2 chamber after eight weeks of treatment and the other groups will be euthanized when they are 300 days old. The prostate will be dissected, removed and processed according to the histological, immunohistochemistry and Western blotting techniques for p63/RACEMASE, AR, Er±, Er², NOX1, Nrf2, TLR4, NFº², TNF-± e IL-6 detection. The immunohistochemistry results will be analyzed and photographed by a light microscopy. The Western blotting results will be performed by the bands semiquantitative densitometry. The morphological and stereological results will be evaluated by statistical analysis. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, TALITA DE MELLO; RIBEIRO DA SILVEIRA, LIVIA TERESA; RINALDI, JAQUELINE CARVALHO; SCARANO, WELLERSON RODRIGO; DOMENICONI, RAQUEL FANTIN. Alterations in prostate morphogenesis in male rat offspring after maternal exposure to Di-n-butyl-phthalate (DBP). REPRODUCTIVE TOXICOLOGY, v. 69, p. 254-264, APR 2017. (13/07203-4)

DE MELO NETO, JOAO SIMAO; GOMES, FABIANA DE CAMPOS; FELIPE PINHEIRO, PATRICIA FERNANDA; PEREIRA, SERGIO; SCARANO, WELLERSON RODRIGO; FAVARO, WAGNER JOSE; DOMENICONI, RAQUEL FANTIN. The effects of high doses of nandrolone decanoate and exercise on prostate microvasculature of adult and older rats. Life Sciences, v. 121, p. 16-21, JAN 15 2015. (13/07203-4)

GOMES, F. DE C.; CHUFFA, L. G. DE A.; FAVARO, W. J.; SCARANO, W. R.; DE MELO-NETO, J. S.; PINHEIRO, P. F. F.; DOMENICONI, R. F.. Nandrolone decanoate and resistance exercise affect prostate morphology and hormone receptor interface in adult rats with implications for the aging process. ANDROLOGY, v. 8, n. 1, p. 211-220, JAN 2020. (13/00649-7, 13/07203-4)

ARAUJO, M. V. T.; SPADELLA, M. A.; CHIES, A. B.; ARRUDA, V, G.; SANTOS, T. DE M.; CAVARIANI, M. M.; DOMENICONI, R. F.. Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland. TISSUE & CELL, v. 53, p. 104-110, AUG 2018. (13/07203-4)

GOMES, F. C.; CHUFFA, L. G. A.; SCARANO, W. R.; PINHEIRO, P. F. F.; FAVARO, W. J.; DOMENICONI, R. F.. Nandrolone decanoate and resistance exercise training favor the occurrence of lesions and activate the inflammatory response in the ventral prostate. ANDROLOGY, v. 4, n. 3, p. 473-480, MAY 2016. (13/07203-4, 13/00649-7)