Veterinary drugs: development and validation of bioanalytical methods using LC-MS/MS and MALDI-TOF-MS and its applications in sanitary surveillance and the development of fish farming in Brazil

• Grant number: 13/50452-5
• Support Opportunities: Research Grants - Research Partnership for Technological Innovation - PITE
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Agreement: Agilent
• Principal Investigator: Felix Guillermo Reyes Reyes
• Grantee: Felix Guillermo Reyes Reyes
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Company: Agilent Technologies Brasil Ltda
• City: Campinas

Abstract

Analytical methods using mass spectrometry detection technique (LC-MS/MS and MALDI-TOF-MS) will be developed and validated for the determination of veterinary drugs residues in fish. In this context, chromatographic methods coupled with mass spectrometry have been successfully applied. Thus, in this project, Identification and / or quantification of veterinary drugs will also be studied using the MALDI-TOF-MS (Matrix-Assisted Laser Desorption / lonization - Time of Flight Mass Spectrometry) technique. MALDI-TOF-MS technique is a well-known tool mainly used for analysis of biomolecules such as proteins, but has timidly been employed in the analysis of small molecules, such as the veterinary drugs used for the treatment of fish diseases. Thus, scientific studies to verify the application of this technique in the determination of veterinary drugs residues in foods must be of great value to the scientific community, regulators and industry. The methods developed and validated must be directed to the determination of an analyte in different matrices, as well as different analytes in a matrix (multiresidue method). Validation will be performed considering the official guides of the European Union (EC 2002) and the Brazilian Ministry of Agriculture, Livestock and Food Supply (MAPA (MAPA 2012). In addition to the publication of scientific papers, validated methods will be transferred through practical training courses, to officers of official laboratories that carrying out this type of analysis, such as The National Plan for Control of Residues and Contaminants (PNCRC/MAPA) and the States Laboratories Public Health such as the Instituto Adolfo Lutz (IAL). Thus, we intend to strengthen action and scientific competence of laboratories in the official surveillance of veterinary drug residues in food for human consumption, in particular, food from fish farming. On the other hand, although Brazil has a tremendous potential for the development of aquaculture, there are only three veterinary drugs licensed for use in this agricultural activity, being two antibiotics which the active principles are florfenicol and oxytetracycline, and antiparasitic based trichlorfon. The lack of therapeutic alternatives has forced producers to use in fish, drugs legislated for other animal species or even chemotherapy not legislated (prohibited), endangering the entire production chain, due to environmental hazards and public health order, so as the ability to exportation. Thus, the validated methods will be used to solve safety feeding aspects in fish farming in Brazil. The development of analytical methods are simple, fast and with a minimum use of organic solvents for the determination of residues of veterinary drugs in fish and its derivatives, using the techniques LC-MS/MS and MALDI-TOF-MS, as well as studies to aid the development of alternative therapies in the national fish is innovative and will provide the reduction of losses related to the lack of quality of final products (processed fish and fish products) and at the same time, reduce the indiscriminate use of antibiotics and antiparasitic drugs in the productive chain. Knowing and controlling the appropriate use of veterinary drugs in fish farming and waste levels of these substances in fish is a prerequisite for compliance and certification of products aimed at the consumer's health and environment, marketing and exportation. (AU)

Scientific publications

(21)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NUNES, KATIA S. D.; ASSALIN, MARCIA R.; VALLIM, JOSE H.; JONSSON, CLAUDIO M.; QUEIROZ, SONIA C. N.; REYES, FELIX G. R.. Multiresidue Method for Quantification of Sulfonamides and Trimethoprim in Tilapia Fillet by Liquid Chromatography Coupled to Quadrupole Time-of-Flight Mass Spectrometry Using QuEChERS for Sample Preparation. JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, 2018. (13/50452-5)

BORTOLOTTE, ANDREA RENATA; DANIEL, DANIELA; DE CAMPOS BRAGA, PATRICIA APARECIDA; REYES REYES, FELIX GUILLERMO. A simple and high-throughput method for multiresidue and multiclass quantitation of antimicrobials in pangasius (Pangasionodon hypophthalmus) fillet by liquid chromatography coupled with tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1124, p. 17-25, AUG 15 2019. (13/50452-5)

SAMPAIO, FERNANDA GARCIA; CARRA, MARIA LIDIA; JONSSON, CLAUDIO MARTIN; GONCALVES, VITORIA TEODORO; DAL'BO, GENOEFA; DAMACENA NUNES, KATIA SANTOS; VALIM, JOSE HENRIQUE; LIMA DALLAGO, BRUNO STEFANO; DO NASCIMENTO DE QUEIROZ, SONIA CLAUDIA; REYES REYES, FELIX GUILLERMO. Effects of Dietary Exposure to Sulfamethazine on the Hematological Parameters and Hepatic Oxidative Stress Biomarkers in Nile Tilapia (Oreochromis niloticus). Bulletin of Environmental Contamination and Toxicology, v. 97, n. 4, p. 528-535, OCT 2016. (13/50452-5)

CRUZ, MICHELLE DEL BIANCHI A.; FERNANDES, MARIA ANGELA M.; MONTEIRO, ALDA LUCIA G.; TELES, JULIANA A.; ANADON, ARTURO; REYES, FELIX G. R.. Tissue residue depletion of moxidectin in lambs (Ovis aries) following subcuianeous administration. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, v. 35, n. 7, p. 1278-1285, 2018. (13/50452-5)

CRUZ, MICHELLE DEL BIANCHI A.; FERNANDES, MARIA A. M.; BRAGA, PATRICIA A. DE C.; MONTEIRO, ALDA L. G.; DANIEL, DANIELA; REYES, FELIX G. R.. Moxidectin residues in lamb tissues: Development and validation of analytical method by UHPLC-MS/MS. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1072, p. 390-396, JAN 1 2018. (13/50452-5)

NUNES, KATIA S. D.; VALLIM, JOSE H.; ASSALIN, MARCIA R.; QUEIROZ, SONIA C. N.; PARAIBA, LOURIVAL C.; JONSSON, CLAUDIO M.; REYES, FELIX G. R.. Depletion study, withdrawal period calculation and bioaccumulation of sulfamethazine in tilapia (Oreochromis niloticus) treated with medicated feed. Chemosphere, v. 197, p. 89-95, APR 2018. (13/50452-5)

CASTELLO BRANCO, LUCIANA; RODRIGUES, MARILI VILLA NOVA; REYES, FELIX GUILLERMO REYES. Effect of food processing (fish burger preparation and frying) on residual levels of enrofloxacin and ciprofloxacin. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, v. 38, n. 10, JUL 2021. (13/50452-5)

BRAGA, PATRICIA A. DE C.; EBERLIN, MARCOS N.; REYES, FELIX G. R.. Applicability of MALDI-TOF MS for determination of quinolone residues in fish. Journal of Mass Spectrometry, v. 54, n. 12, p. 1008-1012, DEC 2019. (13/50452-5)

CORDEIRO, RAFAELLE PEREIRA; DE CAMPOS BRAGA, PATRICIA APARECIDA; JONSSON, CLAUDIO MARTIN; BRANDAO, FRANMIR RODRIGUES; CHAGAS, EDSANDRA CAMPOS; REYES REYES, FELIX GUILLERMO. herapeutic efficacy and bioaccumulation of albendazole in the treatment of tambaqui (Colossoma macropomum) parasitized by acanthocephalan (Neoechinorhynchus buttnerae. AQUACULTURE RESEARCH, v. 53, n. 4, NOV 2021. (13/50452-5)

BORTOLOTTE, ANDREA RENATA; DANIEL, DANIELA; REYES, FELIX GUILLERMO REYES. Occurrence of antimicrobial residues in tilapia (Oreochromis niloticus) fillets produced in Brazil and available at the retail market. Food Research International, v. 140, FEB 2021. (13/50452-5)

NUNES, KATIA S. D.; ASSALIN, MARCIA R.; VALLIM, JOSE H.; JONSSON, CLAUDIO M.; QUEIROZ, SONIA C. N.; REYES, FELIX G. R.. Multiresidue Method for Quantification of Sulfonamides and Trimethoprim in Tilapia Fillet by Liquid Chromatography Coupled to Quadrupole Time-of-Flight Mass Spectrometry Using QuEChERS for Sample Preparation. JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, v. 2018, p. 10-pg., 2018-01-01. (13/50452-5)

RAFAELLE P. CORDEIRO; PATRÍCIA A. DE C. BRAGA; CLAUDIO M. JONSSON; FRANMIR R. BRANDÃO; EDSANDRA C. CHAGAS; FELIX G. R. REYES. Efeito do revestimento com etilcelulose de ração medicada contendo albendazol sobre a eficácia do tratamento da acantocefalose em tambaqui (Colossoma macropomum) naturalmente parasitado. Acta Amazonica, v. 54, n. 2, 2024-02-23. (13/50452-5)

SOUZA, ANA PAULA FERREIRADE; PETRARCA, MATEUS HENRIQUE; DE CAMPOS BRAGA, PATRICIA APARECIDA; RODRIGUES, NADIA REGINA; REYES, FELIX GUILLERMO REYES. nalysis of insecticide residues in honey by liquid chromatography tandem mass spectrometry using QuEChERS optimized by the Plackett Burman desig. CYTA-JOURNAL OF FOOD, v. 19, n. 1, p. 326-332, JAN 1 2021. (13/50452-5)

BARRETO, FABIOLA M.; DA SILVA, MARIANA R.; BRAGA, PATRICIA A. C.; BRAGOTTO, ADRIANA P. A.; HISANO, HAMILTON; REYES, FELIX G. R.. Evaluation of the leaching of florfenicol from coated medicated fish feed into water. Environmental Pollution, v. 242, n. B, p. 1245-1252, NOV 2018. (13/50452-5)

CASTELLO BRANCO, LUCIANA; RODRIGUES, MARILI VILLA NOVA; REYES, FELIX GUILLERMO REYES. Effect of food processing (fish burger preparation and frying) on residual levels of enrofloxacin and ciprofloxacin. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, JUL 2021. (13/50452-5)

HOGA, CELIA A.; ALMEIDA, FERNANDA L.; REYES, FELIX G. R.. A review on the use of hormones in fish farming: Analytical methods to determine their residues. CYTA-JOURNAL OF FOOD, v. 16, n. 1, p. 679-691, 2018. (13/50452-5)

HOGA, CELIA A.; RECHE, KARINE V. G.; ALMEIDA, FERNANDA L.; REIS, VANESSA R.; CORDEIRO, RAFAELLE P.; ANADON, ARTURO; REYES, FELIX G. R.. Development and validation of an analytical method for the determination of 17 beta-estradiol residues in muscle of tambaqui (Colossoma macropomum Cuvier, 1818) by LC-MS/MS and its application in samples from a fish sexual reversion study. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1128, n. SI, OCT 1 2019. (13/50452-5)

SOUZA, ANA PAULA FERREIRADE; PETRARCA, MATEUS HENRIQUE; DE CAMPOS BRAGA, PATRICIA APARECIDA; RODRIGUES, NADIA REGINA; REYES, FELIX GUILLERMO REYES. Analysis of insecticide residues in honey by liquid chromatography tandem mass spectrometry using QuEChERS optimized by the Plackett Burman design. CYTA-JOURNAL OF FOOD, v. 19, n. 1, p. 7-pg., 2021-01-01. (13/50452-5)

BARRETO, FABIOLA M.; DA SILVA, MARIANA R.; BRAGA, PATRICIA A. C.; BRAGOTTO, ADRIANA P. A.; HISANO, HAMILTON; REYES, FELIX G. R.. Evaluation of the leaching of florfenicol from coated medicated fish feed into water. Environmental Pollution, v. 242, p. 8-pg., 2018-11-01. (13/50452-5)

CORDEIRO, RAFAELLE PEREIRA; DE CAMPOS BRAGA, PATRICIA APARECIDA; SOUZA ROCHA, MARIA JULIETE; CHAGAS, EDSANDRA CAMPOS; REYES REYES, FELIX GUILLERMO. Depletion study and estimation of the withdrawal period for albendazole in tambaqui (Colossoma macropomum) parasitised by acanthocephalan (Neoechinorhynchus buttnerae) treated with albendazole-containing feed. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, v. 38, n. 11, SEP 2021. (13/50452-5)

MACHADO FERNANDES, MARIA ANGELA; GILAVERTE, SUSANA; BIANCHI, MICHELE DELL; ARAUJO DA SILVA, CLAUDIO JOSE; MOLENTO, MARCELO BELTRAO; REYES REYES, FELIX GUILLERMO; GOMES MONTEIRO, ALDA LUCIA. Moxidectin residues in tissues of lambs submitted to three endoparasite control programs. Research in Veterinary Science, v. 114, p. 406-411, OCT 2017. (13/50452-5)