Evaluation of insulin sensitivity and insulin signal transduction in adult rats with pulp disease

• Grant number: 12/08722-2
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2013
• End date: April 30, 2016
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Doris Hissako Matsushita
• Grantee: Doris Hissako Matsushita
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The focal oral inflammation can cause deleterious systemic manifestations in the human body, such as diabetes mellitus (DM). This fact proves to be relevant because surgical interventions conducted in diabetic patients (with higher glycemia) involve serious risk to health. The pulp lesion (PL) is characterized by local inflammation and increased amounts of cytokines such as TNF and IL-6 which are known to induce cellular resistance to insulin. The inflammation associated with insulin deficiency causes physiological changes that confer immunosuppression, reducing the number of osteoblasts and consequent decreased ability to repair tissue in the individual. However, a correlation between DM and LP is still unknown. Aware that the inflammatory cytokines TNF-alfa and IL-6 may generate alterations in insulin signal, it becomes essential to investigate the possibility of LP, which is a local inflammatory process, "per se" to cause insulin resistance in nondiabetic individuals. The aim of this study will be to assess in rats with LP: 1)glycemia, insulinemia, cholesterolemia, triacilgliceridemia and plasma concentrations of inflammatory cytokines (TNF-alfa and IL-6), 2) insulin sensitivity, 3) the pp185 (IRS1/IRS2)phosphorylation status in tyrosine and in serine in periepididymal white adipose tissue (WAT), in skeletal muscle gastrocnemius (G) and in liver (L), 4) the serine phosphorylation status of protein kinase B (PKB or Akt) in WAT , in G and in L. For this study, 60 rats (2-month-old) will be used and will be divided into two groups: a) control rats without LP b) rats with LP whose disease will be induced in right molars employing carbon steel ball drill (on the end with 0.1 mm). After 30 days of LP induction, the animals will be anesthetized for the experiments. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ASTOLPHI, RAFAEL DIAS; CURBETE, MARIANE MACHADO; CHIBA, FERNANDO YAMAMOTO; ANGELO CINTRA, LUCIANO TAVARES; ERVOLINO, EDILSON; DE OLIVEIRA DA MOTA, MAX SANDER; ANTONIALI, CRISTINA; SALIBA GARBIN, CLEA ADAS; SUMIDA, DORIS HISSAKO. Periapical Lesions Decrease Insulin Signaling in Rat Skeletal Muscle. JOURNAL OF ENDODONTICS, v. 41, n. 8, p. 1305-1310, AUG 2015. (12/08722-2)