Studies on persisting infections by human respiratory Paramyxoviruses

• Grant number: 13/16349-2
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2013
• End date: May 31, 2016
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Eurico de Arruda Neto
• Grantee: Eurico de Arruda Neto
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Edwin Tamashiro ; Wilma Terezinha Anselmo Lima

Abstract

Infections caused by human paramyxoviruses have great impact in public health, but little is known about their pathogenesis. Evidence recently produced by our group suggests that these agents frequently persist in lymphoid tissue of human tonsils. Such tonsillar persistence of paramyxoviruses is importante because of the possible pathogenic and immunologic consequences, as well as because of the potential impact of asymptomatic carriers shedding paramyxovirus in the community. The present project, strongly based on preliminary data, aims at investigating the persistence of a respiratory paramyxoviroma in lymphoid tonsillar tissues, shedding light on mechanisms of this persistence, an area virtually unexplored in human virology. We expect to confirm presence of replication markers of human respiratory syncytial virus (HRSV), human metapneumovirus (HMPV) and human parainfluenza virus (HPIV). The paramyxoviral RNA profiling will generate the polyssomal active transcriptome of these agents. The host cell phenotypes and intracelular localization of replication will be determined. Also, the presence of paramyxoviral functional proteins diffused beyond the infected cells will be assessed. We will also develop a fluorescent HRSV clone by reverse genetics and establish ex vivo and in vivo models of HRSV infection. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, RONALDO BRAGANCA; ROCHA, LUCAS PENNA; PRATES, MIRELA MOREIRA; GAGLIARDI, TALITA BIANCA; BIASOLI, BALDUINO; LEITE, MARCELO JUNQUEIRA; BUZATTO, GUILHERME; HYPPOLITO, MIGUEL ANGELO; ARAGON, DAVI CASALE; TAMASHIRO, EDWIN; et al. Respiratory DNA viruses are undetectable in nasopharyngeal secretions from adenotonsillectomized children. PLoS One, v. 12, n. 3, MAR 17 2017. (13/16349-2)

CARDOSO, RICARDO S.; TAVARES, LUCAS ALVES; JESUS, BRUNA LAIS S.; CRIADO, MIRIA F.; DE CARVALHO, ANDREIA NOGUEIRA; SOUZA, JULIANO DE PAULA; BEDI, SUKHMANI; DE SOUZA, MARCOS MICHEL; SILVA, MARIA LUCIA; LANFREDI, GUILHERME PAUPERIO; et al. Host Retromer Protein Sorting Nexin 2 Interacts with Human Respiratory Syncytial Virus Structural Proteins and is Required for Efficient Viral Production. MBIO, v. 11, n. 5, SEP-OCT 2020. (13/16349-2)

CARDOSO, RICARDO DE SOUZA; MENDES VIANA, ROSA MARIA; VITTI, BRENDA CRISTINA; LUNARDELLO COELHO, ANA CAROLINA; SANTOS DE JESUS, BRUNA LAIS; SOUZA, JULIANO DE PAULA; PONTELLI, MARJORIE CORNEJO; MURAKAMI, TOMOYUKI; VENTURA, ARMANDO MORAIS; ONO, AKIRA; et al. Human Respiratory Syncytial Virus Infection in a Human T Cell Line Is Hampered at Multiple Steps. Viruses-Basel, v. 13, n. 2, FEB 2021. (13/16349-2)

PONTELLI, MARJORIE C.; CASTRO, ITALO A.; MARTINS, RONALDO B.; LA SERRA, LEONARDO; VERAS, FLAVIO P.; NASCIMENTO, DANIELE C.; SILVA, CAMILA M.; CARDOSO, RICARDO S.; ROSALES, ROBERTA; GOMES, ROGERIO; et al. SARS-CoV-2 productively infects primary human immune system cells in vitro and in COVID-19 patients. JOURNAL OF MOLECULAR CELL BIOLOGY, v. 14, n. 4, p. 14-pg., 2022-08-17. (14/02438-6, 13/16349-2)