Research of genes involved in the resistance to carbapenems and monobactams in environmental isolates of Pseudomonas aeruginosa

• Grant number: 13/19401-5
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2013
• End date: November 30, 2015
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Eliana Guedes Stehling
• Grantee: Eliana Guedes Stehling
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Bacteria belonging to the genus Pseudomonas have a great metabolic versatility, a wide capacity to adapt, can inhabit in various environments and acquire resistance to a considerable variety of antimicrobials. One of the mechanisms of acquired resistance to the carbapenems meropenem and imipenem, in Pseudomonas aeruginosa, is by the production of the enzime metallo-²-lactamase (MBL) and the resistance to the monobactam aztreonam is mainly caused by altering the permeability of the drug through changes in the bacteria outer membrane and by a system of ejection through efflux pump of the type MexAB-OprM.This research project aims to study the profile and mechanisms of antimicrobial resistance to some ²-lactamics of the carbapenems (imipenem and meropenem) and monobactams (aztreonam) classes in P. aeruginosa isolated from soil of the five Brazilian regions. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRAZ, VANIA S.; FURLAN, JOAO PEDRO R.; FERNANDES, ANA FLAVIA T.; STEHLING, ELIANA G.. Mutations in NalC induce MexAB-OprM overexpression resulting in high level of aztreonam resistance in environmental isolates of Pseudomonas aeruginosa. FEMS Microbiology Letters, v. 363, n. 16, AUG 2016. (13/19401-5, 14/02806-5)

PITONDO-SILVA, ANDRE; DEVECHIO, BEATRIZ BAPTISTELLA; SILVA MORETTO, JESSICA APARECIDA; STEHLING, ELIANA GUEDES. High prevalence of bla(VIM-1) gene in bacteria from Brazilian soil. Canadian Journal of Microbiology, v. 62, n. 10, p. 820-826, OCT 2016. (13/19401-5)

SILVA MORETTO, JESSICA APARECIDA; ALTARUGIO, LUCAS MIGUEL; ANDRADE, PEDRO AVELINO; FACHIN, ANA LUCIA; ANDREOTE, FERNANDO DINI; STEHLING, ELIANA GUEDES. Changes in bacterial community after application of three different herbicides. FEMS Microbiology Letters, v. 364, n. 13, JUL 2017. (13/19401-5)

PITONDO-SILVA, ANDRE; MARTINS, VINICIUS VICENTE; STEHLING, ELIANA GUEDES. First report of the bla(VIM) gene in environmental isolates of Buttiauxella sp.. APMIS, v. 123, n. 4, p. 326-329, APR 2015. (13/19401-5)

FERNANDES, ANA FLAVIA TONELLI; PARTATA DA SILVA, MICHELLE BARBOSA; MARTINS, VINICIUS VICENTE; SARAIVA MIRANDA, CARLOS EDUARDO; STEHLING, ELIANA GUEDES. Isolation and characterization of a Pseudomonas aeruginosa from a virgin Brazilian Amazon region with potential to degrade atrazine. Environmental Science and Pollution Research, v. 21, n. 24, p. 13974-13978, DEC 2014. (13/19401-5, 09/16657-3)

PITONDO-SILVA, ANDRE; GONCALVES, GUILHERME BARTOLOMEU; STEHLING, ELIANA GUEDES. Heavy metal resistance and virulence profile in Pseudomonas aeruginosa isolated from Brazilian soils. APMIS, v. 124, n. 8, p. 681-688, AUG 2016. (13/19401-5)