Proteomic/Glycoproteomic characterization of the venoms of the Bothrops jararaca complex with emphasis on the N-terminome and N-glycome of toxins

• Grant number: 13/13548-4
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2014
• End date: October 31, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Solange Maria de Toledo Serrano
• Grantee: Solange Maria de Toledo Serrano
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The comprehensive knowledge of venom proteome complexity of Bothrops species is important because these snakes cause most accidents in Brazil. Besides ecological and taxonomical implications, knowledge of the natural history and toxin composition of venoms is of fundamental importance for the treatment of envenomation and in the selection of specimens for the preparation of venom pools for antivenom production. The Jararaca complex is composed of the nominal species Bothrops jararaca, which has a broad geographical distribution in the Southeastern region of Brazil including some islands, and two insular species (B. insularis and B. alcatraz) as well as the recently described B. otavioi species. Venoms of the Bothrops genus cause a complex pathological envenomation, with local tissue damage and systemic effects on the hemostatic and cardiovascular systems. Variability in the venom proteome/peptidome is a well-documented phenomenon. It occurs at several levels including intergenus, interspecies, and intraspecies variation. Moreover, venom composition may be influenced by the geographical origin and habitat of the snake. Despite the use of various high throughput approaches in toxinology studies, the natural proteome of snake venoms, i.e. the diversity of toxins that is present in the venoms in native form just after synthesis/secretion by the venom gland cells, is poorly known. Likewise, little is known about the post-translational modifications (PTM) present in toxins of Bothrops venoms and how they vary among species. Glycosylation is a frequent protein PTM and considering that a number of toxins in Bothrops venoms are glycosylated it is reasonable to assume that they could display a role in human envenomation cases. The main goal of this study is to use mass spectrometric approaches to analyze the N-terminal sequences of proteins and peptides (natural N-terminome) present in the venoms of B. jararaca and B. alcatraz as well as their glycoproteome and N-glycome, in order to understand the complex evolution process of these species. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZELANIS, ANDRE; SILVA, DEBORA A.; KITANO, EDUARDO S.; LIBERATO, TARCISIO; FUKUSHIMA, ISABELLA; SERRANO, SOLANGE M. T.; TASHIMA, ALEXANDRE K.. A first step towards building spectral libraries as complementary tools for snake venom proteome/peptidome studies. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 31, SEP 2019. (13/14651-3, 14/06579-3, 17/20106-9, 13/07467-1, 17/07897-7, 13/13548-4)

ANDRADE-SILVA, DEBORA; ZELANIS, ANDRE; KITANO, EDUARDO S.; JUNQUEIRA-DE-AZEVEDO, INACIO L. M.; REIS, MARCELO S.; LOPES, ALINE S.; SERRANO, SOLANGE M. T.. Proteomic and Glycoproteomic Profilings Reveal That Post- translational Modifications of Toxins Contribute to Venom Phenotype in Snakes. JOURNAL OF PROTEOME RESEARCH, v. 15, n. 8, p. 2658-2675, AUG 2016. (13/07467-1, 13/13548-4)

SANTORO, MARCELO LARAMI; DO CARMO, THAIS; LOPES CUNHA, BRUNA HELOISA; ALVES, ANDRE FONSECA; ZELANIS, ANDRE; DE TOLEDO SERRANO, SOLANGE MARIA; GREGO, KATHLEEN FERNANDES; SANT'ANNA, SAVIO STEFANINI; BARBARO, KATIA CRISTINA; FERNANDES, WILSON. Ontogenetic Variation in Biological Activities of Venoms from Hybrids between Bothrops erythromelas and Bothrops neuwiedi Snakes. PLoS One, v. 10, n. 12, DEC 29 2015. (13/07467-1, 10/08162-1, 13/13548-4, 08/02996-8, 09/15413-3, 13/25177-0)

ANDRADE-SILVA, DEBORA; ZELANIS, ANDRE; TRAVAGLIA-CARDOSO, SILVIA R.; NISHIYAMA, JR., MILTON Y.; SERRANO, SOLANGE M. T.. Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates. JOURNAL OF PROTEOME RESEARCH, v. 20, n. 2, p. 1341-1358, FEB 5 2021. (13/13548-4, 16/16935-7, 13/07467-1, 13/14651-3)

BRAS-COSTA, CAROLINA; CHAVES, ALISON FELIPE ALENCAR; CAJADO-CARVALHO, DANIELA; DA SILVA PIRES, DAVID; ANDRADE-SILVA, DEBORA; SERRANO, SOLANGE M. T.. Profilings of subproteomes of lectin-binding proteins of nine Bothrops venoms reveal variability driven by different glycan types. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1870, n. 7, p. 15-pg., 2022-07-01. (15/23017-1, 17/09929-3, 13/13548-4)

ZELANIS, ANDRE; MENEZES, MILENE C.; KITANO, EDUARDO S.; LIBERATO, TARCISIO; TASHIMA, ALEXANDRE K.; PINTO, ANTONIO F. M.; SHERMAN, NICHOLAS E.; HO, PAULO L.; FOX, JAY W.; SERRANO, SOLANGE M. T.. Proteomic identification of gender molecular markers in Bothrops jararaca venom. JOURNAL OF PROTEOMICS, v. 139, p. 26-37, APR 29 2016. (13/07467-1, 13/13548-4, 98/14307-9)

BRAS-COSTA, CAROLINA; CHAVES, ALISON FELIPE ALENCAR; TREVISAN-SILVA, DILZA; MENEZES, MILENE C.; ROCHA, MARISA M. T.; CAJADO-CARVALHO, DANIELA; ANDRADE-SILVA, DEBORA; SERRANO, SOLANGE M. T.. Sialic acid-containing glycans play a role in the activity of snake venom proteases. Biochimie, v. 204, p. 14-pg., 2023-01-07. (13/13548-4, 13/07467-1, 15/23017-1, 17/09929-3)

ANDRADE-SILVA, DEBORA; ASHLINE, DAVID; TRAN, THUY; LOPES, ALINE SORIANO; TRAVAGLIA CARDOSO, SILVIA REGINA; REIS, MARCELO DA SILVA; ZELANIS, ANDRE; SERRANO, SOLANGE M. T.; REINHOLD, VERNON. Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes. MOLECULAR & CELLULAR PROTEOMICS, v. 17, n. 7, p. 1261-1284, JUL 2018. (13/07467-1, 14/12245-0, 13/14651-3, 13/13548-4)

GREN, ERIC C. K.; KITANO, EDUARDO S.; ANDRADE-SILVA, DEBORA; IWAI, LEO KEI; REIS, MARCELO S.; MENEZES, MILENE C.; SERRANO, SOLANGE M. T.. Comparative analysis of the high molecular mass subproteomes of eight Bothrops snake venoms. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 30, p. 113-121, JUN 2019. (13/07467-1, 15/01234-0, 13/13548-4)