In vivo evaluation of lipoteichoic acid, endotoxin and microrganisms in necrotic teeth

• Grant number: 12/11287-6
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2014
• End date: January 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Cláudio Antonio Talge Carvalho
• Grantee: Cláudio Antonio Talge Carvalho
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
• City of the host institution: São José dos Campos
• Associated researchers: Frederico Canato Martinho ; Luciane Dias de Oliveira

Abstract

The present study will evaluate the levels of lipoteichoic acid (LTA ), endotoxins and microorganisms in root canals with pulp necrosis and radiographically visible periapical lesion during treatment with endodontic instrumentation mechanized and 2.5% sodium hypochlorite. In addition, there will be a correlation between the amount of LTA, endotoxins, microbial organisms, periapical lesion size and symptomatology. 20 uni-root teeth with necrotic pulp will be instrumented with the system of alternating rotation Reciproc and irrigated with 2.5% sodium hypochlorite. Samples will be collected of root canal immediately after opening coronary after biomechanical preparation, after using trisodium EDTA and 17% after 14 days of action from the dressing of calcium hydroxide with 2% chlorhexidine gel. For all collections will be performed the following tests: identification and quantification of microorganisms by Real Time PCR, quantification of endotoxin testing by kinetic chromogenic lysate from amoebocytes of Limulus and quantification of lipoteichoic acid by enzyme immunoassay (ELISA). The periapical lesions will be measured in early endodontic treatment, for later correlation with the amount of endotoxins, lipoteichoic acid and symptomatology. The data will be statistically analyzed using the Kruskall-Wallis, Dunn (5% significance level) and Linear Correlation of Pearson. (AU)