Genetic and epigenetic factors in the etiology of the cutaneous melanoma

• Grant number: 13/07480-8
• Support type: Regular Research Grants
• Duration: February 01, 2014 - January 31, 2016
• Field of knowledge: Biological Sciences - Genetics
• Principal Investigator: Ana Cristina Victorino Krepischi
• Grantee: Ana Cristina Victorino Krepischi
• Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Assoc. researchers: Dirce Maria Carraro ; Helena Paula Brentani ; João Pedreira Duprat Neto ; Maria Isabel Alves de Souza Waddington Achatz

Abstract

The cutaneous melanoma is a rare skin cancer highly aggressive that accounts for up to 75% of the deaths by cutaneous tumors. The majority is sporadic but about 10% of the cases occurs in individuals carrying a germinative mutation. There are two main known melanoma predisposition genes exhibiting highly penetrant mutations: CDKN2A and CDK4, both of them also somatically inactivated in sporadic melanomas. However, CDKN2A mutations (mainly affecting p16) are detected in only 20-40% of the hereditary cutaneous melanoma - characterized by familial aggregation and/or multiple primary melanomas - indicating that a great proportion of the melanoma susceptibility has no known genetic etiology. This scenario indicates either the existence of susceptibility genes yet not revealed or alternative mechanisms to point mutations.Rare genomic deletions and duplications, and genetic variants conferring intermediate cancer risk can also increase the cancer susceptibility; recently, studies have suggested that germinative epimutations (such as aberrant DNA methylation pattern) can provoke cancer predisposition. The identification of the etiology of the melanoma susceptibility is crucial not only to the ascertaining of individuals with a high cancer risk but also to the widening of the tumor biology understanding; susceptibility cancer genes often are also mutated in sporadic tumors. Therefore, the research on the genome and epigenome of patients and their tumors can aid in the identification of molecular pathways involved in the genesis and progression of the cutaneous melanoma that could be future targets for intervention.The main objective of this project is the identification of novel genetic and epigenetic factors associated to the susceptibility and development of cutaneous melanomas. The experimental design to achieving this goal is: (a) investigation of the genome (mainly CNV analysis, and TERT mutation screening) and epigenome (CDKN2A promotor methylation and global DNA methylation for a subset)of a group of patients with high melanoma predisposition who are negative for CDKN2A/CDK4 mutations; (b) study of the methylome of 30 cutaneous melanoma aiming to establish correlation with the mutational profile of the genes NRAS, BRAF, KIT, and TERT. Following the analysis of these data, genes will be selected for validation of their relevance in the cutanelus melanoma. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BONALDI, ADRIANO; KASHIWABARA, ANDRE; DE ARAUJO, ERICA S.; PEREIRA, LYGIA V.; PASCHOAL, ALEXANDRE R.; ANDOZIA, MAYRA B.; VILLELA, DARINE; RIVAS, MARIA P.; SUEMOTO, CLAUDIA K.; PASQUALUCCI, CARLOS A.; GRINBERG, LEA T.; BRENTANI, HELENA; MARIA-ENGLER, SILVYA S.; CARRARO, DIRCE M.; VIANNA-MORGANTE, ANGELA M.; ROSENBERG, CARLA; VASQUES, LUCIANA R.; KREPISCHI, ANA. Mining Novel Candidate Imprinted Genes Using Genome-Wide Methylation Screening and Literature Review. EPIGENOMES, v. 1, n. 2 SEP 2017. Web of Science Citations: 1.

DE ARAUJO, ERICA S. S.; KASHIWABARA, ANDRE Y.; ACHATZ, MARIA I. W.; MOREDO, LUCIANA F.; DE SA, BIANCA C. S.; DUPRAT, JOAO P.; ROSENBERG, CARLA; CARRARO, DIRCE M.; KREPISCHI, ANA C. V. LINE-1 hypermethylation in peripheral blood of cutaneous melanoma patients is associated with metastasis. Melanoma Research, v. 25, n. 2, p. 173-177, APR 2015. Web of Science Citations: 11.

DE ARAUJO, ERICA S. S.; PRAMIO, DIMITRIUS T.; KASHIWABARA, ANDRE Y.; PENNACCHI, PAULA C.; MARIA-ENGLER, SILVYA S.; ACHATZ, MARIA I.; CAMPOS, ANTONIO H. J. F. M.; DUPRAT, JOAO P.; ROSENBERG, CARLA; CARRARO, DIRCE M.; KREPISCHI, ANA C. V. DNA Methylation Levels of Melanoma Risk Genes Are Associated with Clinical Characteristics of Melanoma Patients. BIOMED RESEARCH INTERNATIONAL, 2015. Web of Science Citations: 4.

SOUZA DE ARAUJO, ERICA SARA; MARCHI, FABIO ALBUQUERQUE; RODRIGUES, TATIANE CRISTINA; VIEIRA, HENRIQUE CURSINO; KUASNE, HELLEN; WADDINGTON ACHATZ, MARIA ISABEL; MOREDO, LUCIANA FACURE; SOARES DE SA, BIANCA COSTA; DUPRAT, JOAO PEREIRA; BRENTANI, HELENA PAULA; ROSENBERG, CARLA; CARRARO, DIRCE MARIA; VICTORINO KREPISCHI, ANA CRISTINA. Genome-wide DNA methylation profile of leukocytes from melanoma patients with and without CDKN2A mutations. Experimental and Molecular Pathology, v. 97, n. 3, p. 425-432, DEC 2014. Web of Science Citations: 3.

FIDALGO, FELIPE; GOMES, ELIMAR ELIAS; FACURE, LUCIANA MOREDO; DA SILVA, FELIPE CARNEIRO; CARRARO, DIRCE MARIA; SOARES DE SA, BIANCA COSTA; DUPRAT NETO, JOAO PEDREIRA; VICTORINO KREPISCHI, ANA CRISTINA. Association of melanoma with intraepithelial neoplasia of the pancreas in three patients. Experimental and Molecular Pathology, v. 97, n. 1, p. 144-147, AUG 2014. Web of Science Citations: 2.