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Conjunctival swab to Real Time PCR detection of canine visceral leishmaniasis in epidemiological surveys


Canine visceral leishmaniasis is a severe zoonosis caused by Leishmania chagasi (syn.infantum). The life cycle of this parasite and transmission occurs primarily through the bite of the female vector, the dipteran Lutzomyia longipapis. Domestic dog is the main source of infection for the vector in the urban area, due to the high prevalence of the disease in this species and high degree of parasitism skin, facilitating vector infection. As consequence, dogs are the major targets of human disease control. The Ministry of Health adopts serological tests to positive animal's detection; however sensitivity and specificity of these tests are questionable. In the last decade molecular toolsproved to be great allies in the detection of leishmaniasis, and have high sensitivity and specificity. A variety of biological specimens may be used in these tests, such as blood and bone marrow aspirate.Conjunctival swab is an easy and practical alternative to obtain biological samples and are noninvasive. Recent work has tested conjunctival swab efficacy for leishmaniasis detection by PCR, and concluded that their use is of great value in diagnosis. With real-time PCR or quantitative PCR(qPCR) technology, is possible to increase PCR sensitivity, specificity and reproducibility. Besides, this technique, allows quantify the parasitic load in infected animal. In this project, the use of realtime PCR will be evaluated by comparing it with IFA and common PCR. It is hoped that comparing mentioned techniques, a more accurate and sensitive diagnostic test can be found, which can be safely used in epidemiological surveys to detect canine leishmaniasis (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
JOÃO AUGUSTO FRANCO LEONEL; BRUNA TANNIHÃO; JULIA ASSIS ARANTES; GEOVANNA VIOTI; JULIA CRISTINA BENASSI; ROBERTA ARIBONI BRANDI; HELENA LAGE FERREIRA; LARA BORGES KEID; RODRIGO MARTINS SOARES; TRÍCIA MARIA FERREIRA DE SOUSA OLIVEIRA. Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR. Revista do Instituto de Medicina Tropical de São Paulo, v. 63, . (13/19821-4)
BENASSI, JULIA C.; BENVENGA, GRAZIELLA U.; FERREIRA, HELENA L.; SOARES, RODRIGO M.; SILVA, DIOGO T.; PEREIRA, VANESSA F.; RUIZ, VERA LETTICIE A.; OLIVEIRA, TRICIA MARIA F. DE S.. Detecção molecular e sorológica de infecção por Leishmania (Leishmania) infantum em cavalos na região Sudeste do Brasil. Pesquisa Veterinária Brasileira, v. 38, n. 6, p. 1058-1063, . (11/00147-6, 13/19821-4)

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