Advanced search
Start date
Betweenand

Conjunctival swab to Real Time PCR detection of canine visceral leishmaniasis in epidemiological surveys

Grant number: 13/19821-4
Support type:Regular Research Grants
Duration: February 01, 2014 - January 31, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Trícia Maria Ferreira de Sousa Oliveira
Grantee:Trícia Maria Ferreira de Sousa Oliveira
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Assoc. researchers:Helena Lage Ferreira

Abstract

Canine visceral leishmaniasis is a severe zoonosis caused by Leishmania chagasi (syn.infantum). The life cycle of this parasite and transmission occurs primarily through the bite of the female vector, the dipteran Lutzomyia longipapis. Domestic dog is the main source of infection for the vector in the urban area, due to the high prevalence of the disease in this species and high degree of parasitism skin, facilitating vector infection. As consequence, dogs are the major targets of human disease control. The Ministry of Health adopts serological tests to positive animal's detection; however sensitivity and specificity of these tests are questionable. In the last decade molecular toolsproved to be great allies in the detection of leishmaniasis, and have high sensitivity and specificity. A variety of biological specimens may be used in these tests, such as blood and bone marrow aspirate.Conjunctival swab is an easy and practical alternative to obtain biological samples and are noninvasive. Recent work has tested conjunctival swab efficacy for leishmaniasis detection by PCR, and concluded that their use is of great value in diagnosis. With real-time PCR or quantitative PCR(qPCR) technology, is possible to increase PCR sensitivity, specificity and reproducibility. Besides, this technique, allows quantify the parasitic load in infected animal. In this project, the use of realtime PCR will be evaluated by comparing it with IFA and common PCR. It is hoped that comparing mentioned techniques, a more accurate and sensitive diagnostic test can be found, which can be safely used in epidemiological surveys to detect canine leishmaniasis (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BENASSI, JULIA C.; BENVENGA, GRAZIELLA U.; FERREIRA, HELENA L.; SOARES, RODRIGO M.; SILVA, DIOGO T.; PEREIRA, VANESSA F.; RUIZ, VERA LETTICIE A.; OLIVEIRA, TRICIA MARIA F. DE S. Molecular and serological detection of Leishmania spp. in horses from an endemic area for canine visceral leishmaniasis in southeastern Brazil. Pesquisa Veterinária Brasileira, v. 38, n. 6, p. 1058-1063, JUN 2018. Web of Science Citations: 2.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.