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Right ventricular hypertrophy after pulmonary artery banding in rats: alterations structure, function and genetic


Introduction and objectives: The right ventricle hypertrophy and remodeling may occur in some congenital and acquired heart diseases. The molecular mechanisms and the evolution are not well known. Also, the knowledge about the alterations of the left ventricle associated with right ventricular remodeling is poor. So, the present research project have the following objectives: to analyze the evolution of the morphological, functional and microscopic parameters, as well as genetic aspects involved in the process of adaptation of right ventricle caused by pulmonary artery banding in rats; to analyze the influences of right ventricle remodeling in the left ventricle geometry, mechanics, morphology, histology and performance; and to characterize the echocardiographic parameters of the right cardiac chambers that can predict the type of remodeling and the development of heart failure after pulmonary artery banding.Methods:Wistar-EPM rats with 6 weeks and weigh ranging from 170 to 200 grams. One day after initial echocardiogram the animals underwent to pulmonary artery banding or not (sham animals). The animals of both groups were divided in 4 subgroups according to duration of follow up: 2, 4, 6 or 8 weeks, when a new echocardiogram was performed followed by hemodynamic study. After this, we obtained myocardial tissue from right and left ventricle for histology (hypertrophy and fibrosis) and molecular biology (genetic). To analyze the data we plane to use descriptive statics. ANOVA (two-way) or Kruskal-Wallis tests will be used for comparison of the morphological, echocardiographic and molecular data among the different duration of pulmonary artery banding. To compare each variable between the subgroups t test or Willcoxon will be used, and linear regression to analyze the relation between lung and liver water content and echocardiographic variables. The significance level was defined as 5% (p<0.05). (AU)