Right ventricular hypertrophy after pulmonary artery banding in rats: alterations structure, function and genetic

• Grant number: 13/05111-5
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2014
• End date: January 31, 2016
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Valdir Ambrósio Moises
• Grantee: Valdir Ambrósio Moises
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Andrey Jorge Serra ; José Antonio Silva Junior ; Paulo Jose Ferreira Tucci

Abstract

The right ventricle hypertrophy and remodeling may occur in some congenital and acquired heart diseases. The molecular mechanisms and the evolution are not well known. Also, the knowledge about the alterations of the left ventricle associated with right ventricular remodeling is poor. So, the present research project have the following objectives: to analyze the evolution of the morphological, functional and microscopic parameters, as well as genetic aspects involved in the process of adaptation of right ventricle caused by pulmonary artery banding in rats; to analyze the influences of right ventricle remodeling in the left ventricle geometry, mechanics, morphology, histology and performance; and to characterize the echocardiographic parameters of the right cardiac chambers that can predict the type of remodeling and the development of heart failure after pulmonary artery banding. Methods: Wistar-EPM rats with 6 weeks and weigh ranging from 170 to 200 grams. One day after initial echocardiogram, the animals underwent to pulmonary artery banding or not (sham animals). The animals of both groups were divided in 4 subgroups according to duration of follow up: 2, 4, 6 or 8 weeks, when a new echocardiogram was performed followed by hemodynamic study. After this, we obtained myocardial tissue from right and left ventricle for histology (hypertrophy and fibrosis) and molecular biology (genetic). To analyze the data we plane to use descriptive statics. ANOVA (two-way) or Kruskal-Wallis tests will be used for comparison of the morphological, echocardiographic and molecular data among the different duration of pulmonary artery banding. To compare each variable between the subgroups, t test or Willcoxon will be used, and linear regression to analyze the relation between lung and liver water content and echocardiographic variables. The significance level was defined as 5% (p<0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, JAIRO MONTEMOR AUGUSTO; ANTONIO, EDNEI LUIZ; DOS SANTOS, LUIS FELIPE NEVES; SERRA, ANDREY JORGE; FELICIANO, REGIANE SANTOS; JUNIOR, JOSE ANTONIO SILVA; IHARA, SILVIA SAIULI MIKI; TUCCI, PAULO JOSE FERREIRA; MOISES, VALDIR AMBROSIO. Hypertrophy of the right ventricle by pulmonary artery banding in rats: a study of structural, functional, and transcriptomics alterations in the right and left ventricles. FRONTIERS IN PHYSIOLOGY, v. 14, p. 17-pg., 2023-07-27. (13/05111-5)