| Grant number: | 13/21621-3 |
| Support Opportunities: | Regular Research Grants |
| Start date: | March 01, 2014 |
| End date: | February 28, 2017 |
| Field of knowledge: | Health Sciences - Physiotherapy and Occupational Therapy |
| Principal Investigator: | Thiago Luiz de Russo |
| Grantee: | Thiago Luiz de Russo |
| Host Institution: | Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil |
Abstract
Stroke is considered the mainly cause of disability in the world. One of the most important complaints of individuals post-stroke is the upper limp disability. The taskspecific training is largerly used by the rehabilitation team to treat post-stroke subjects. However the molecular mechanisms involved on skeletal muscle adaptation due to task specific training remain unknown. Thus, the main objective of this study is to assess the effect of task-specific training, throughout time, on morphological and molecular adaptation of the skeletal muscle of forelimb in post-stroke gerbils. Both paretic and non-paretic limbs will be investigated. Hundred forty male adult gerbils (Meriones unguiculatus), 2-3 month, will be submitted to unilateral common carotid artery occlusion for 10 minutes using a thread of suture followed by reperfusion. The taskspecific training will be performed during 15 days, beginning 24 hours after surgery, by single pellet-retrieval task. Functionality will be analyzed by cylinder test and single pellet-retrieval task on the day before surgery procedure and, then, 6, 13 and 20 days after surgery. Sham groups will be performed to comparision effects to training and control groups. Animals will be euthanized at 7, 14 or 21 days. Morphometric (muscle fiber cross sectional area) and morphologic analyses will be performed on biceps brachii muscles in both forelimbs. The protein content of atrophy enzymes, such as MuRF1, atrogin-1 and Fbxo40, and also of muscle mass control, myostatin, and transcription, as MyoD, will be quantified by Western Blot. For statistical analysis, data will be submitted to homogeneity (Levene) and normality (Shapiro-Wilk) tests. In case of parametric data, Anova one-way followed by Tukey test will be performed. Repeated-Measures ANOVA will be performed for functional data. Significance level will set at 5%. (AU)
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