Development of a database for molecular profile management of commercial cultivars, parents and wild accessions of sugarcane

• Grant number: 13/22500-5
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Start date: March 01, 2014
• End date: February 29, 2016
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Luciana Rossini Pinto Machado da Silva
• Grantee: Luciana Rossini Pinto Machado da Silva
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil

Abstract

The efficient management and utilization of the germplasm of a breeding program is critical to the development of new cultivars. In Brazil, the sugarcane breeding programs have used molecular markers to characterize the molecular profile of their genotypes, however, there is a lack of a consolidated bank with the molecular profile of cultivars, elite clones and wild accessions. The Sugarcane Breeding Program from the Agronomic Institute developed the software (Sugarprint) which is capable of storing either the profile of sugarcane genotypes generated by molecular markers or based on the molecular profile of an unknown genotype find the corresponding cultivar previously stored in the bank. For the effective use and implementation of this software it is necessary to establish the molecular profile of the genotypes of interest. This project has as main objective start the construction of a molecular profile database of sugarcane cultivars, wild accessions and elite clones used as parents in crosses. In addition, we intend to create a database of genomic DNA of these genotypes; identify a set of SSR primer pairs with high discriminatory power; estimate the genetic variability within and between the groups assessed and develop SCAR markers (Sequence Characterized Amplified Regions) derived from AFLP (Amplified Fragment Length Polymorphism) species-specific to S. spontaneum. The development of SCAR markers will allow the rapid identification of hybrids derived from crosses between sugarcane commercial cultivars and accessions of S. spontaneum assisting the Genetic Introgression Bioenergy Programs. We emphasize that, to our knowledge, until the present moment, in the case of public sugarcane breeding programs, in Brazil does not exist, a molecular profile database of such nature to sugarcane. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, ALESSANDRA ALVES; DA SILVA, MARCEL F.; PINTO, LUCIANA ROSSINI. Epigenetic diversity of Saccharum spp. accessions assessed by methylation-sensitive amplification polymorphism (MSAP). 3 BIOTECH, v. 10, n. 6, MAY 24 2020. (13/22500-5)

MARTINS, ALESSANDRA ALVES; DA SILVA, MARCEL F.; PINTO, LUCIANA ROSSINI. Epigenetic diversity of Saccharum spp. accessions assessed by methylation-sensitive amplification polymorphism (MSAP). 3 BIOTECH, v. 10, n. 6, p. 14-pg., 2020-05-24. (13/22500-5)

NEUBER, ANA CAROLINE; CAMILO DOS SANTOS, FERNANDA RAQUEL; DA COSTA, JULIANA BORGES; VOLPIN, MAICON; XAVIER, MAURO ALEXANDRE; PERECIN, DILERMANDO; VILLAVICENCIO BURBANO, ROBERTO CARLOS; DE ANDRADE LANDELL, MARCOS GUIMARAES; PINTO, LUCIANA ROSSINI. Survey of the Bru1 gene for brown rust resistance in Brazilian local and basic sugarcane germplasm. PLANT BREEDING, v. 136, n. 2, p. 182-187, APR 2017. (13/22500-5)

VIEIRA MANECHINI, JOAO RICARDO; DA COSTA, JULIANA BORGES; PEREIRA, BRUNA TURCATTO; CARLINI-GARCIA, LUCIANA APARECIDA; XAVIER, MAURO ALEXANDRE; DE ANDRADE LANDELL, MARCOS GUIMARAES; PINTO, LUCIANA ROSSINI. Unraveling the genetic structure of Brazilian commercial sugarcane cultivars through microsatellite markers. PLoS One, v. 13, n. 4, APR 23 2018. (13/22500-5, 08/57908-6)