Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups

• Grant number: 14/01603-3
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: March 01, 2014
• End date: August 31, 2014
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Luciana Pellegrini Pisani
• Grantee: Luciana Pellegrini Pisani
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil

Abstract

To evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring. On the first day of pregnancy rats were divided into four groups: control diet (C), C supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or T supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. At 21th, pups were weighed and length was measured. Serum concentrations of LPS and free fatty acids (FFA) were performed. Bacterial DNA of the faces was determined by real-time PCR. Data were expressed as mean ± standard error and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p< 0.05 was significant. The oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF compared to C and T respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. of the CF in relation to C. In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups. (AU)