Investigation of low molecular weight seaweeds sulfated polysaccharides on the structure and function of secretory phospholipase A2 Crotalus durissus terrificus

• Grant number: 13/10938-6
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2014
• End date: July 31, 2016
• Field of knowledge: Health Sciences - Pharmacy - Pharmacognosy
• Principal Investigator: Marcos Hikari Toyama
• Grantee: Marcos Hikari Toyama
• Host Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil

Abstract

Prospected compounds from marine organisms has received great attention in recent years as a promising new area of study in several areas, ranging from the development of compounds for paints with application in the shipbuilding industry to cosmeceuticals related products, especially those related to creams and has gone down into other areas since the pharmacology of anti microbial (Jiao et al., 2011). Natural compounds from algae are therefore considered to be a promising new frontier and poorly investigated. Among these new compounds are sulfated polysaccharides from seaweed and low molecular weight oligosaccharides related marine macroalgae who displayed a range of biological activities: anticoagulant and antithrombotic activities (Pomin et al., 2008), antiviral activity (Witvrouw and Clercq, 1997 ), immunomodulatory activity (Chen et al., 2008), antioxidant activity (Rocha de Souza et al., 2007), activity antilipidemic (Pengzhan et al., 2003) and more recently modulating activity of secretory phospholipase A2 (Toyama et al ., 2010 and Pires et al., 2013) and anti leishmania (Pires et al., 2013). Therefore there is a great perceptive on the use of these compounds on several areas of research related to bioprospection new bioactive compounds for inflammation or other areas. In recent years, our group has been dedicated to investigating the effects of sulfated polysaccharides from seaweed on the pro-inflammatory effects, enzymatic and myotoxic induced secretory Phospholipase A2 and snake on Leishmaniasis shows how the results summarized in Table I.All compounds are listed in Table I were fractionated in our laboratory as well as determination of their characteristics shown. From these results, we chose the following sulfated polysaccharides of algae for the presentation of the proposed project and this choice is due to the fact that we have developed in collaboration with the research group in macroalgae Federal University of Fortaleza and fractionation protocols in laboratory scale and can standardize obtaining sulphated polysaccharides of low molecular weight, especially with a molecular mass of about 10 kDa Codium isthmocladum, Caulerpa racemosa and Caulerpa sertularioides. Therefore, as we have isolated and these polysaccharides with molecular weights as defined by mass spectrometry MALDI-TOFF, we want to investigate the molecular nature that maintains molecular estability of the heterodimers of sPLA2: Ps in solution, to investigate using spectroscopic techniques for measure possible changes induced Ps from Codium isthmocladum, Caulerpa racemosa and Caulerpa sertularioides on the secondary and tertiary structure of sPLA2 and try to form co-crystals sPLA2: Ps for future research studies using X-ray crystallography to determine the molecular structure of the sulfated polysaccharide and the molecular regions these compounds involved in the interaction with sPLA2 and investigate the effect of prior administration of these compound in mice on edema and myotoxicity induced sPLA2 and completing studies and publish results that others already have. In addition, we intend to investigate the effect of these three sulfated polysaccharides on the modulation and expression of COX 1 and COX 2 and LOX. In this project, therefore, we intend to evaluate and enhance or refute on the molecular basis, using sPLA2 as a model of molecular target for the use of these compounds for the exploration of potential new herbal medicines against inflammation induced secretory sPLA2. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BITTENCOURT RODRIGUES, CAROLINE FABRI; PENA FERREIRA, MARCELO JOSE; BELCHOR, MARIANA NOVO; COSTA, CAROLINE R. C.; NOVAES, DANIELLE P.; DOS SANTOS JUNIOR, ADEILSO BISPO; TAMAYOSE, CINTHIA I.; TERASHIMA PINHO, MARCUS VINICIUS; DE OLIVEIRA, MARCOS ANTONIO; TOYAMA, MARCOS HIKARI. Evaluation of the Inhibitory Potential of Casuarictin, an Ellagitannin Isolated from White Mangrove (Laguncularia racemosa) Leaves, on Snake Venom Secretory Phospholipase A2. MARINE DRUGS, v. 17, n. 7, JUL 2019. (17/20291-0, 14/20932-8, 14/21593-2, 13/10938-6)

BITTENCOURT RODRIGUES, CAROLINE FABRI; GAETA, HENRIQUE HESSEL; BELCHOR, MARIANA NOVO; PENA FERREIRA, MARCELO JOSE; TERASHIMA PINHO, MARCUS VINICIUS; TOYAMA, DANIELA DE OLIVEIRA; TOYAMA, MARCOS HIKARI. Evaluation of Potential Thrombin Inhibitors from the White Mangrove (Laguncularia racemosa (L.) C.F. Gaertn.). MARINE DRUGS, v. 13, n. 7, p. 4505-4519, JUL 2015. (13/10938-6)