Skin healing under treatment with monoterpene (menthol) and triterpene (Lupeol) in experimental model

• Grant number: 13/23340-1
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2014
• End date: May 31, 2016
• Field of knowledge: Biological Sciences - Pharmacology - Ethnopharmacology
• Principal Investigator: Claudia Helena Pellizzon
• Grantee: Claudia Helena Pellizzon
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Ariane Leite Rozza ; Carlos Alberto Hussni

Abstract

The loss of skin integrity due to physical, chemical or thermal injuries results in the formation of a wound. In consequence, the healing process is triggered, reestablishing the function and integrity of the injured skin. The healing process is classified into three overlapping phases: inflammatory (hemostasis and inflammation), proliferative (granulation tissue formation and reepithelization) and extracellular matrix remodeling phase. Menthol and lupeol presents antinociceptive, antiinflammatory and antimicrobial effects. This research project aims to evaluate the healing potential from menthol and lupeol, in topical cream, in cutaneous wounds in rats and horses, elucidating the mechanism of action. Male Wistar rats (n=7) or horses will be distributed into groups: ointment base (control), collagenase cream (positive control), lupeol or menthol ointment at 2.5, 5 and 10% (w/w). After anesthesia, a circular excision of 2 cm of diameter will be created on the dorsal region of the animals. The wounds will be treated and measured daily for 3, 7 or 21 days (according to the phases described in the literature). After euthanasia, histological (inflammatory cells count, inflammatory score, morphology of collagen fibers), histomorphometrical (regenerative epithelium height measurement) and immunohistochemistry (epidermal and vascular endothelial growth factors, cell proliferation, apoptosis and cytoprotection) analyses will be performed, besides molecular analyses (qPCR and RT-PCR for collagen I and III, epidermal and ²1-transforming growth factor). Analyses of antioxidant activity (glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase and myeloperoxidase) will be performed in the liver of the rats and analyses of anti-inflammatory activity (tumor necrosis factor-±, pro-inflammatory interleukins IL-1² and IL-6 and anti-inflammatory interleukin IL-10) will be performed in the rats' serum. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BESERRA, FERNANDO PEREIRA; VIEIRA, ANA JULIA; SERGIO GUSHIKEN, LUCAS FERNANDO; DE SOUZA, EDUARDO OLIVEIRA; HUSSNI, MARIA FERNANDA; HUSSNI, CARLOS ALBERTO; NOBREGA, RAFAEL HENRIQUE; MONTEIRO MARTINEZ, EMANUEL RICARDO; JACKSON, CHRISTOPHER JOHN; DE AZEVEDO MAIA, GABRIELA LEMOS; et al. Lupeol, a Dietary Triterpene, Enhances Wound Healing in Streptozotocin-Induced Hyperglycemic Rats with Modulatory Effects on Inflammation, Oxidative Stress, and Angiogenesis. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2019. (13/23340-1, 14/23247-4, 17/17600-1)

ROZZA, ARIANE LEITE; BESERRA, FERNANDO PEREIRA; VIEIRA, ANA JULIA; OLIVEIRA DE SOUZA, EDUARDO; HUSSNI, CARLOS ALBERTO; MARTINEZ, EMANUEL RICARDO MONTEIRO; NOBREGA, RAFAEL HENRIQUE; PELLIZZON, CLAUDIA HELENA. The Use of Menthol in Skin Wound Healing-Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization. PHARMACEUTICS, v. 13, n. 11, NOV 2021. (13/23340-1, 13/22202-4, 15/06636-0, 16/13307-5)

BESERRA, FERNANDO PEREIRA; VIEIRA, ANA JULIA; SERGIO GUSHIKEN, LUCAS FERNANDO; DE SOUZA, EDUARDO OLIVEIRA; HUSSNI, MARIA FERNANDA; HUSSNI, CARLOS ALBERTO; NOBREGA, RAFAEL HENRIQUE; MONTEIRO MARTINEZ, EMANUEL RICARDO; JACKSON, CHRISTOPHER JOHN; DE AZEVEDO MAIA, GABRIELA LEMOS; et al. Lupeol, a Dietary Triterpene, Enhances Wound Healing in Streptozotocin-Induced Hyperglycemic Rats with Modulatory Effects on Inflammation, Oxidative Stress, and Angiogenesis. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2019, p. 20-pg., 2019-01-01. (14/23247-4, 17/17600-1, 13/23340-1)

BESERRA, FERNANDO PEREIRA; SERGIO GUSHIKEN, LUCAS FERNANDO; VIEIRA, ANA JULIA; BERGAMO, DANILO AUGUSTO; BERGAMO, PATRICIA LUISA; DE SOUZA, MARIANA OLIVEIRA; HUSSNI, CARLOS ALBERTO; TAKAHIRA, REGINA KIOMI; NOBREGA, RAFAEL HENRIQUE; MONTEIRO MARTINEZ, EMANUEL RICARDO; et al. From Inflammation to Cutaneous Repair: Topical Application of Lupeol Improves Skin Wound Healing in Rats by Modulating the Cytokine Levels, NF-kappa B, Ki-67, Growth Factor Expression, and Distribution of Collagen Fibers. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 14, JUL 2020. (13/23340-1, 14/23247-4, 17/17600-1)