multi-user equipment approved in grant 2012/15165-2: flow cytometry Accuri

• Grant number: 14/07171-8
• Support Opportunities: Multi-user Equipment Program
• Start date: June 01, 2014
• End date: May 31, 2017
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Rodolfo de Paula Vieira
• Grantee: Rodolfo de Paula Vieira
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated research grant: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals, AP.JP
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Processos Biológicos - Caracterização - Contagem células (inclui citômetros)
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

Asthma is a chronic airway inflammatory disease that results in impairment of quality of life. In the last years, several studies have demonstrated that aerobic training (AT) performed properly results in anti-inflammatory effects to the airways, either in experimental models of asthma and to asthmatic individuals. However, although few cellular effects have been investigated (still only in animals' models), the possible extra and intracellular mediators underlying the effects of AT on asthma never were investigated. Thus, the present project aims to evaluate the effects of AT on purinergic signaling and also of SOCS, JAK and STAT proteins signaling on the lungs and lymphatic organs (thymus, spleen and bone marrow) as possible modulators of the anti-inflammatory effects of aerobic training in two experimental models of asthma (by using ovalbumin - OVA and house dust mite - HDM). In addition, this project also aims to evaluate the total and differential cell number in induce sputum, the expression of purinergic receptors and the expression and phosphorilation of SOCS, JAK and STATs in the cells obtained from induced sputum of asthmatic individuals submitted to aerobic training, as well as the levels of inflammatory mediators in the supernatant of induced sputum and also in the condensate of exhaled air. In addition, the antigen presentation by dendritic cells to CD4+ T lymphocytes obtained from peripheral blood of asthmatic patients submitted to rehabilitation will be evaluated. The response of CD4+ T lymphocytes from peripheral blood (proliferation and cytokines production in vitro) will be additionally evaluated. Therefore, this project may present for the first time a real link between basic/experimental with clinical research, concerning the effects of aerobic training for asthma. Then, using two experimental asthma models (OVA and HDM), the animals will be sensitized and challenged and then submitted to aerobic training. 24h after the last session of training and allergen challenge, the animals will be assessed to the inflammatory profile in the bronchoalveolar lavage and in the lung tissue, to the levels of pro-inflammatory cytokines (Th2 cytokines - IL-5 and IL-13 and TNF-alpha) and to the levels of anti-inflammatory cytokines (IL-10, IL-1ra) and also for the expression of sTNFR1 and sTNFR2, as well as for the airway remodeling, pulmonary mechanics and bronchial hyperresponsiveness. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA RIGONI, VERA LUCIA; KWASNIEWSKI, FABIO H.; VIEIRA, RODOLFO PAULA; LINHARES, INGRID SESTREM; VEIGA DA SILVA, JOELMIR LUCENA; NOGUEIRA-PEDRO, AMANDA; ZAMUNER, STELLA REGINA. Human bronchial epithelial cells injury and cytokine production induced by Tityus serrulatus scorpion venom: An in vitro study. Toxicon, v. 120, p. 22-28, SEP 15 2016. (12/15165-2, 14/07171-8)