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Multi-User Equipment approved in grant 2014/07328-4. Coordinator: Profa Sandra Helena Poliselli Farsky. Equipment: flow cytometer Accuri

Grant number: 15/07812-6
Support type:Multi-user Equipment Program
Duration: August 01, 2015 - August 31, 2017
Field of knowledge:Biological Sciences - Pharmacology - General Pharmacology
Principal researcher:Sandra Helena Poliselli Farsky
Grantee:Sandra Helena Poliselli Farsky
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:14/07328-4 - Identification of endogenous pathways for the control of inflammation, AP.TEM
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Type of equipment:Processos Biológicos - Caracterização - Contagem células (inclui citômetros)
Manufacturer: Fabricante não informado
Model: Modelo não informado

Abstract

The flow cytometer is a device that separates the cells by size and granulosity, marking membrane, cytoplasmic and nuclear proteins, using specific fluorophores. In the present project, the flow cytometry will be used for quantification of cell death and oxidative stress, expression of adhesion molecules, Annexin A1, TSPO, besides the secretion of cytokines by the use of CBAs kits. For its wide range of employment, as the diversity of cell types and proteins that can be analyzed, the flow cytometer will be employed by other researchers of FCF-USP and / or other units of USP, which have research project in hematology, neoplasms, immunology and genotoxicology, parasitology, among others. (AU)