Multi-User Equipment approved in grant 2014/07328-4. Coordinator: Profa Sandra Helena Poliselli Farsky. Equipment: flow cytometer Accuri

• Grant number: 15/07812-6
• Support Opportunities: Multi-user Equipment Program
• Start date: August 01, 2015
• End date: August 31, 2017
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Sandra Helena Poliselli Farsky
• Grantee: Sandra Helena Poliselli Farsky
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 14/07328-4 - Identification of endogenous pathways for the control of inflammation, AP.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Processos Biológicos - Caracterização - Contagem células (inclui citômetros)
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The flow cytometer is a device that separates the cells by size and granulosity, marking membrane, cytoplasmic and nuclear proteins, using specific fluorophores. In the present project, the flow cytometry will be used for quantification of cell death and oxidative stress, expression of adhesion molecules, Annexin A1, TSPO, besides the secretion of cytokines by the use of CBAs kits. For its wide range of employment, as the diversity of cell types and proteins that can be analyzed, the flow cytometer will be employed by other researchers of FCF-USP and / or other units of USP, which have research project in hematology, neoplasms, immunology and genotoxicology, parasitology, among others. (AU)