Identification of miRNAs involved in the modulation of angiogenesis-related genes by polyphenols

• Grant number: 14/50238-6
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2014
• End date: June 30, 2016
• Field of knowledge: Health Sciences - Pharmacy
• Agreement: Universidad de la Frontera
• Principal Investigator: Dulcineia Saes Parra Abdalla
• Grantee: Dulcineia Saes Parra Abdalla
• Principal researcher abroad: Luis Antonio Salazar Navarrete
• Institution abroad: Universidad de La Frontera (UFRO), Chile
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 12/51316-5 - Study of the activity of biodrugs, PPARs agonists and natural products with therapeutic potential in atherosclerosis, AP.TEM

Abstract

In order to identify those microRNAs involved in the modulation of angiogenesis-related genes that was observed after treatment with polyphenols from propolis, different cell types participating in the formation of atherosclerotic plaque will be used. In macrophages, endothelial cells and smooth muscle cells, the expression of pro-angiogenic factors and microRNAs modulated by treatment with polyphenols from propolis, previously identified from in vivo experiments using LDLR knockout mice, will be characterized. The expression profile of genes and microRNAs before and after treatment with propolis polyphenols will be compared to identify genes and microRNAs modulated in each specific cell type. Finally, the expression profile of angiogenesis-related genes after polyphenols treatment will be assessed post blocking individual microRNAs using an anti-miR oligonucleotide, permitting to identify those microRNAs that are important for maintain the described modulation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAAVEDRA, NICOLAS; CUEVAS, ALEJANDRO; CAVALCANTE, MARCELA F.; DOERR, FELIPE A.; SAAVEDRA, KATHLEEN; ZAMBRANO, TOMAS; ABDALLA, DULCINEIA S. P.; SALAZAR, LUIS A.. Polyphenols from Chilean Propolis and Pinocembrin Reduce MMP-9 Gene Expression and Activity in Activated Macrophages. BIOMED RESEARCH INTERNATIONAL, 2016. (14/50238-6)

SAAVEDRA, NICOLAS; CUEVAS, ALEJANDRO; CAVALCANTE, MARCELA F.; DOERR, FELIPE A.; SAAVEDRA, KATHLEEN; ZAMBRANO, TOMAS; ABDALLA, DULCINEIA S. P.; SALAZAR, LUIS A.. Polyphenols from Chilean Propolis and Pinocembrin Reduce MMP-9 Gene Expression and Activity in Activated Macrophages. BIOMED RESEARCH INTERNATIONAL, v. 2016, p. 8-pg., 2016-01-01. (14/50238-6)