Validation of a cost-effective strategy for tracking rare erythrocyte and platelet phenotypes aiming to organize a rare blood donor program in São Paulo

• Grant number: 14/50250-6
• Support Opportunities: Research Grants - Research in Public Policies for the National Health Care System (PP-SUS)
• Start date: July 01, 2014
• End date: January 31, 2016
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Agreement: CNPq - PPSUS
• Principal Investigator: Ester Cerdeira Sabino
• Grantee: Ester Cerdeira Sabino
• Host Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Goals: 1- Validate a high-throughput genotyping strategy using OpenArray® equipment to determine most important erythrocyte and pletelet antigens, in order to make this methodology suitable for tracking rare donors, 2- Elaborate a customized software; to register the donors and to compile all genotyping data. 3- Molecular characterization of sickle cell patients with RhD/RhCE variants, in order to provide compatible blood units. Methods: Automated DNA extraction of 5000 repeat donors. Genotyping using Openarray® equipment for 32 alleles which codify erythrocyte and platelet antigens (MNS, Lutheran, Kell, Duffy, Kidd, Diego, Cartwright, Dombrock, Colton, XK, Cromer, KNOPS, JMH, HPA 1-5 e 15). Complete interfacing of the data to a customized software.Next generation sequencing (IonTorrent) of RhD and RhCE genes of sickle cell patients with serologic suspected variants. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DINARDO, CARLA L.; KELLY, SHANNON; DEZAN, MARCIA R.; RIBEIRO, INGRID H.; CASTILHO, SHIRLEY L.; SCHIMIDT, LUCIANA C.; VALGUEIRO, MARIA DO C.; PREISS, LILIANA R.; CUSTER, BRIAN; SABINO, ESTER C.; et al. Diversity of RH and transfusion support in Brazilian sickle cell disease patients with unexplained Rh antibodies. Transfusion, v. 59, n. 10, AUG 2019. (14/50250-6)

DEZAN, MARCIA R.; RIBEIRO, INGRID HELENA; OLIVEIRA, VALERIA B.; VIEIRA, JULIANA B.; GOMES, FRANCISCO C.; FRANCO, LUCAS A. M.; VARUZZA, LEONARDO; RIBEIRO, ROBERTO; CHINOCA, KAREN ZIZA; LEVI, JOSE EDUARDO; et al. RHD and RHCE genotyping by next-generation sequencing is an effective strategy to identify molecular variants within sickle cell disease patients. BLOOD CELLS MOLECULES AND DISEASES, v. 65, p. 8-15, JUN 2017. (14/50250-6)

OLIVEIRA, V. B.; DEZAN, M. R.; GOMES, F. C. A.; MENOSI GUALANDRO, S. F.; KRIEGER, J. E.; PEREIRA, A. C.; MARSIGLIA, J. D.; LEVI, J. E.; ROCHA, V.; MENDRONE-JUNIOR, A.; et al. -318C/T polymorphism of the CTLA-4 gene is an independent risk factor for RBC alloimmunization among sickle cell disease patients. INTERNATIONAL JOURNAL OF IMMUNOGENETICS, v. 44, n. 5, p. 219-224, OCT 2017. (14/50250-6)