Use of a polymerase chain reaction technique for Toxocara canis DNA detection in sera of experimentally infected mice

• Grant number: 14/07345-6
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2014
• End date: June 30, 2016
• Field of knowledge: Biological Sciences - Parasitology - Helminthology of Parasites
• Principal Investigator: Susana Angélica Zevallos Lescano
• Grantee: Susana Angélica Zevallos Lescano
• Host Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Fabiana Martins de Paula ; Pedro Paulo Chieffi

Abstract

Toxocariasis is a zoonosis of public health significance, caused by infection with embryonated eggs of Toxocara canis or T. cati. In humans, different types of clinical syndromes have been identified: Visceral larva migrans (VLM), ocular larva migrans (OLM), Covert toxocariasis (CT) and sometimes neurotoxocariasis (NT). Because being a paratenic host, the parasite does not develop in man to the adult stage so it is not possible to detect it in parasitological examination, unless tissue biopsy. The immunological techniques have shown good sensitivity, however in regions where occurrence of infections by other enteroparasitic helminthes is frequent, previous absorption of sera with antigens of those helminthes is necessary aiming to prevent cross reactions. On the other hand, do not permit specific differentiation, fact that would be resolved using molecular techniques. To evaluate the use of blood samples in molecular diagnostics, in this experimental model, BALB / c mice with different doses of embryonated eggs of T. canis will be used and evaluated in the acute and chronic phases of the parasitism. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GABRIELA RODRIGUES E FONSECA; MARCELO ANDREETTA CORRAL; FABIANA MARTINS DE PAULA; DIRCE MARY CORREIA LIMA MEISEL; RONALDO CESAR BORGES GRYSCHEK; SUSANA ANGÉLICA ZEVALLOS LESCANO. Toxocara canis 30-35 kDa excretory-secretory antigen is an important marker in mice challenged by inocula containing different parasite load levels. Revista do Instituto de Medicina Tropical de São Paulo, v. 64, 2022-02-02. (14/07345-6)

RODRIGUES E FONSECA, GABRIELA; CORRAL, MARCELO ANDREETTA; DE PAULA, FABIANA MARTINS; CORREIA LIMA MEISEL, DIRCE MARY; BORGES GRYSCHEK, RONALDO CESAR; ZEVALLOS LESCANO, SUSANA ANGELICA. oxocara canis 30-35 kDa excretory-secretory antigen is an important marker in mice challenged by inocula containing different parasite load level. Revista do Instituto de Medicina Tropical de São Paulo, v. 64, 2022. (14/07345-6)

RODRIGUES E FONSECA, GABRIELA; DE MELO, GESSICA BAPTISTA; DE PAULA, FABIANA MARTINS; MALTA, FERNANDA MELLO; BORGES GRYSCHEK, RONALDO CESAR; ZEVALLOS LESCANO, SUSANA ANGELICA. Toxocara DNA amplification in serum and tissue samples in BALB/c mice. Molecular and Biochemical Parasitology, v. 246, NOV 2021. (14/07345-6)

GABRIELA RODRIGUES E FONSECA; SERGIO VIEIRA DOS SANTOS; PEDRO PAULO CHIEFFI; FABIANA MARTINS DE PAULA; RONALDO CESAR BORGES GRYSCHEK; SUSANA ANGÉLICA ZEVALLOS LESCANO. Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response. Memórias do Instituto Oswaldo Cruz, v. 112, n. 5, p. 382-386, 2017-03-27. (14/07345-6)