| Grant number: | 15/07627-4 |
| Support Opportunities: | Regular Research Grants |
| Start date: | September 01, 2015 |
| End date: | November 30, 2017 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine |
| Principal Investigator: | Helio Langoni |
| Grantee: | Helio Langoni |
| Host Institution: | Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil |
| Associated researchers: | Sandra de Moraes Gimenes Bosco ; Virgínia Bodelão Richini Pereira |
Abstract
Currently, among the emerging diseases, most are zoonoses, and bats have an important role as source of infection or transmission of infectious agents. Fungal diseases such as histoplasmosis, cryptococcosis, and paracoccidioidomicosis are reported in different species of animals, and their agents can be isolated from grottos, caverns, and animals feces. Rabies can be transmitted by bats and the presence of these animals infected by the rabies virus, in urban areas, represents a risk to the public health, since many of them present synanthropic habits. Thus, the objective of the present study is performing the molecular detection of the fungi Histoplasma capsulatum, Cryptococcus spp. and Paracoccidioidis brasiliensis and the rabies virus in chiropterans in the city of Botucatu and surroundings, in the state of São Paulo. Lung, spleen and liver from chiropterans will be collected for the research and diagnosis of H. capsulatum, C. neoformans and P. brasiliensis using the microbiological cultivation and techniques of molecular diagnosis: Polymerase Chain Reaction (PCR) and Nested-PCR. For the research of the rabies virus, the encephalic material will be collected from the animals and the technique of Direct Immunofluorescence and Inoculation in 21-day old Mice will be performed. Besides, the Polymerase Chain Reaction (PCR), Nested-PCR and Genetic Sequencing of the positive samples will be used. The georeferencing of the samples will be performed with the aid of Arcgis 10.1® software for the generation of maps with the distribution of the animals' collection points. The delimitation of the study will by series of cases. The statistic analysis will be performed by the distribution and frequency of the characteristics relevant to the case, such as: species, feeding habits of bats and place found. Subsequently, it will be submitted to analysis by PROC FREC procedure. (AU)
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