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Molecular research of pathogenic fungi and rabies virus in chiropterans from the region of Botucatu, State of São Paulo, Brazil

Abstract

Currently, among the emerging diseases, most are zoonoses, and bats have an important role as source of infection or transmission of infectious agents. Fungal diseases such as histoplasmosis, cryptococcosis, and paracoccidioidomicosis are reported in different species of animals, and their agents can be isolated from grottos, caverns, and animals feces. Rabies can be transmitted by bats and the presence of these animals infected by the rabies virus, in urban areas, represents a risk to the public health, since many of them present synanthropic habits. Thus, the objective of the present study is performing the molecular detection of the fungi Histoplasma capsulatum, Criptococcus spp. and Paracoccidioidis brasiliensis and the rabies virus in chiropterans in the city of Botucatu and surroundings, in the state of São Paulo. Lung, spleen and liver from chiropterans will be collected for the research and diagnosis of H. capsulatum, C. neoformans and P. brasiliensis using the microbiological cultivation and techniques of molecular diagnosis: Polymerase Chain Reaction (PCR) and Nested-PCR. For the research of the rabies virus, the encephalic material will be collected from the animals and the technique of Direct Immunofluorescence and Inoculation in 21-day old Mice will be performed. Besides, the Polymerase Chain Reaction (PCR), Nested-PCR and Genetic Sequencing of the positive samples will be used. The georeferencing of the samples will be performed with the aid of Arcgis 10.1® software for the generation of maps with the distribution of the animals' collection points. The delimitation of the study will by series of cases. The statistic analysis will be performed by the distribution and frequency of the characteristics relevant to the case, such as: species, feeding habits of bats and place found. Subsequently, it will be submitted to analysis by PROC FREC procedure. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MENOZZI, BENEDITO DONIZETE; DA PAZ, GISELLE SOUZA; PAIZ, LAIS MORAES; GARCES, HANS GARCIA; VASCONCELOS ADORNO, BRUNNA MAYLA; ALMEIDA-SILVA, FERNANDO; ZANCOPE OLIVEIRA, ROSELY MARIA; RICHINI-PEREIRA, VIRGINIA BODELAO; CHECHI, JESSICA LUANA; BAGAGLI, EDUARDO; GIMENES BOSCO, SANDRA DE MORAES; LANGONI, HELIO. Rabies virus and Histoplasma suramericanum coinfection in a bat from southeastern Brazil. ZOONOSES AND PUBLIC HEALTH, v. 67, n. 2, p. 138-147, MAR 2020. Web of Science Citations: 0.
DA PAZ, GISELLE S.; ADORNO, BRUNNA MAYLA V.; RICHINI-PEREIRA, VIRGINIA B.; BOSCO, SANDRA M. G.; LANGONI, HELIO. Infection by Histoplasma capsulatum, Cryptococcus spp. and Paracoccidioides brasiliensis in bats collected in urban areas. TRANSBOUNDARY AND EMERGING DISEASES, v. 65, n. 6, p. 1797-1805, DEC 2018. Web of Science Citations: 0.
MENOZZI, BENEDITO DONIZETE; OLIVEIRA, RAFAEL DE NOVAES; PAIZ, LAIS MORAES; RICHINI-PEREIRA, VIRGINIA BODELAO; LANGONI, HELIO. Antigenic and genotypic characterization of rabies virus isolated from bats (Mammalia: Chiroptera) from municipalities in So Paulo State, Southeastern Brazil. ARCHIVES OF VIROLOGY, v. 162, n. 5, p. 1201-1209, MAY 2017. Web of Science Citations: 2.

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