Preventive effects of chitosan coacervate whey protein on body composition and immuno-metabolic aspect in obese mice

• Grant number: 14/16836-3
• Support Opportunities: Regular Research Grants - Publications - Scientific article
• Start date: September 01, 2014
• End date: February 28, 2015
• Field of knowledge: Biological Sciences - Immunology
• Principal Investigator: Lila Missae Oyama
• Grantee: Lila Missae Oyama
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Background: Functional foods containing bioactive compounds of whey may play an importantrole in prevention and treatment of obesity. The aim of this study was to investigate theprospecting of the biotechnological process of coacervation of whey proteins (CWP) in chitosan,and test its antiobesogenic potential. Methods: CWP (100 mg.kg.day) was administered bygavage in mice with diet-induced obesity for 8 weeks. The animals were divided into four groups:control normocaloric diet gavage with water (C) or coacervate (C-CWP); high fat diet gavagewith water (HF) or coacervate (HF-CWP). Results: The HF-CWP reduced weight gain, serumlipid fractions, and displayed reduced adiposity and insulin levels when compared to the HF-Wgroup. Furthermore, adiponectin was significantly higher in HF-CWP group when compared tothe HF. The level of LPS in HF-W group was significantly higher when compared to HF-CWP.The effects observed for IL-10 showed an inverse correlation between the levels of insulin andglucose in the mesenteric adipose tissue in the HF-CWP group. CWP promoted an increase inboth phosphorylation AMPK and the amount of ATGL in the mesenteric adipose tissue in HFCWPgroup. Conclusion: Supplementation of CWP was able to modulate effects, possibly dueto its the high biological value of proteins. We observed a protective effect of the CWP againstthe metabolic changes triggered by obesity, since that CWP improved the inflammatory milieu ofwhite adipose tissue (AU)