Assessment of genetic polymorphisms on progression to AIDS in patients mono-infected and co-infected with human immunodeficiency virus (HIV) and hepatitis C virus (HCV)

• Grant number: 13/21214-9
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2014
• End date: September 30, 2016
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Rejane Maria Tommasini Grotto
• Grantee: Rejane Maria Tommasini Grotto
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Giovanni Faria Silva ; Lenice Do Rosário de Souza ; Maria Inês de Moura Campos Pardini

Abstract

The human vulnerability to HIV infection is not uniform, virological and host factors are determinants on the risk of transmission and natural progression of the infection.Polymorphisms of KIR genes combined with HLA class I alleles of the patient, are being associated with progression of HIV infection. Several studies have been performed in mono-infected with HIV-1, but little is known about the relationship of these polymorphisms in coinfection HIV/HCV. The purpose of this study is to assess infection the progression by HIV in patients coinfected HIV/HCV, based on clinical parameters and laboratory, correlating with a genetic polymorphisms the host (KIR combined HLA class I), which may be useful for elucidating the natural history of the disease.300 samples will be used to form 3 groups (Group 1: 100 HIV mono-infected (subtype B);Group 2: 100 mono-infected HCV (genotype 1);Group 3: 100 Co-Infected HIV-1 (subtype B)/HCV). Determinations of subtypes (HIV) and genotypes (HCV) will be held from plasma, using RT-PCR and sequencing. The definitions of the KIR gene polymorphisms will be determined by PCR-SSP, and HLA will be accomplished out by sequencing. Clinical and laboratory data, regarding the evolution of HIV infection , will be analyzed from the medical records of patients. Statistical tests of association, parametric and nonparametric tests, Kaplan-Meier, log-rank test and logistic regression will be used for evaluation of the parameters involved in the study. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERRASI, ADRIANA CAMARGO; FERNANDEZ, GEYSSON JAVIER; TOMMASINI GROTTO, REJANE MARIA; SILVA, GIOVANNI FARIA; GONCALVES, JOAO; COSTA, MARINA C.; ENGUITA, FRANCISCO J.; DE MOURA CAMPOS PARDINI, MARIA INES. New LncRNAs in Chronic Hepatitis C progression: from fibrosis to hepatocellular carcinoma. SCIENTIFIC REPORTS, v. 10, n. 1, JUN 18 2020. (13/21214-9)

DE MORAES, LEONARDO NAZARIO; TOMMASINI GROTTO, REJANE MARIA; VALENTE, GUILHERME TARGINO; SAMPAIO, HELOISA DE CARVALHO; MAGRO, ANGELO JOSE; FOGACA, LAUANA; WOLF, IVAN RODRIGO; PERAHIA, DAVID; SILVA, GIOVANNI FARIA; SIMOES, RAFAEL PLANA. A novel molecular mechanism to explain mutations of the HCV protease associated with resistance against covalently bound inhibitors. VIRUS RESEARCH, v. 274, DEC 2019. (13/21214-9)

HEBELER-BARBOSA, FLAVIA; MASSOLINI, VIVIAM MILANEZ; WATANABE, THAIS; SILVA, GIOVANNI FARIA; BARBOSA, ALEXANDRE NAIME; SIMOES, RAFAEL PLANA; FERRASI, ADRIANA CAMARGO; DE ANDRADE ZANOTTO, PAOLO MARINHO; DE MOURA CAMPOS PARDINI, MARIA INES; TOMMASINI GROTTO, REJANE MARIA. Influence of the HIV GWG variant in the HIV infection progression in mono and HCV coinfected patients. MEDICINE, v. 98, n. 29, JUL 2019. (17/07711-0, 13/21214-9)