Production of induced pluripotent stem cells as model studies in rabbits

• Grant number: 13/09392-9
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2014
• End date: June 30, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Carlos Eduardo Ambrósio
• Grantee: Carlos Eduardo Ambrósio
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated researchers: Daniele dos Santos Martins ; Felipe Perecin ; Flávio Vieira Meirelles

Abstract

The induced pluripotent stem cells (iPS) were first reported in 2006 by Takahashi and Yamanaka and has been extensively studied. Through the technique, adult somatic cells acquire behavior very similar to embryonic stem cells, eliminating ethical issues related to the use of such research. However, the mechanisms of iPS cells are not yet fully elucidated. Thus, further research is needed to ensure the safety and effectiveness of their possible use in future therapies. Adipose derived stem cells consists in a type of multipotent mesenchymal stem cell that is highlighted by the ease, speed and security of collection and processing. The rabbit iPS cells from adipose derived stem cells will be produced using the introduction of four transcription factors (Oct3/4, Sox2, c-Myc, and KLF4). Embryonic stem cells of rabbits will be used as a control. The behavior of the cells will be analyzed for pluripotency markers Oct4 and Nanog, alkaline phosphatase activity, ability to differentiate into embryoid bodies in vitro and tumor formation in vivo. From these data, comparative analyzes will be performed between embryonic stem cells, adipose derived stem cells and iPS cells of rabbits. From these data, comparative analysis will be performed between embryonic stem cells, adipose derived stem cells and iPS cells of rabbits. The aim of this study is to establish an adequate protocol to produce iPS cells from adipose derived stem cells of rabbits. We expect to develop a Brazilian technology still unpublished in rabbits, and to create a lineage of iPS cells for future application in cellular therapy. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARDOSO, M. T.; PINHEIRO, A. O.; VIDANE, A. S.; CASALS, J. B.; DE OLIVEIRA, V. C.; GONCALVES, N. J. N.; MARTINS, D. S.; AMBROSIO, C. E.. Characterization of teratogenic potential and gene expression in canine and feline amniotic membrane-derived stem cells. REPRODUCTION IN DOMESTIC ANIMALS, v. 52, n. 2, SI, p. 58-64, APR 2017. (13/23850-0, 13/09392-9)

SOUZA, CAROLINA C.. Immunophenotyping lymphocyte and acute phase proteins in canine X-linked muscular dystrophy. Anais da Academia Brasileira de Ciências, v. 90, n. 3, p. 2977-2990, JUL-SEP 2018. (13/09392-9, 09/02958-1, 11/13030-0, 07/51222-2)

ZOMER, HELENA D.; VIDANE, ATANASIO S.; GONCALVES, NATALIA N.; AMBROSIO, CARLOS E.. Mesenchymal and induced pluripotent stem cells: general insights and clinical perspectives. STEM CELLS AND CLONING-ADVANCES AND APPLICATIONS, v. 8, p. 125-134, 2015. (13/09392-9, 12/04196-4)

ZOMER, HELENA DEBIAZI; ROBALLO, KELLY C. S.; LESSA, THAIS BORGES; BRESSAN, FABIANA FERNANDES; GONCALVES, NATALIA NARDELI; MEIRELLES, FLAVIO VIEIRA; TRENTIN, ANDREA GONCALVES; AMBROSIO, CARLOS EDUARDO. Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research. STEM CELLS AND CLONING-ADVANCES AND APPLICATIONS, v. 11, p. 43-54, 2018. (13/09392-9, 17/21266-0)

VIDANE, A. S.; PINHEIRO, A. O.; CASALS, J. B.; PASSARELLI, D.; HAGE, M. C. F. N. S.; BUENO, R. S.; MARTINS, D. S.; AMBROSIO, C. E.. Transplantation of amniotic membrane-derived multipotent cells ameliorates and delays the progression of chronic kidney disease in cats. REPRODUCTION IN DOMESTIC ANIMALS, v. 52, p. 11-pg., 2017-04-01. (13/09392-9)

JULIANA BARBOSA CASALS; NAIRA CAROLINE GODOY PIERI; KELLY CRISTINE SANTOS ROBALLO; FABIANA FERNANDES BRESSAN; PHELIPE OLIVEIRA FAVARON; DANIELE DOS SANTOS MARTINS; CARLOS EDUARDO AMBRÓSIO. Pluripotent stem cells proliferation is associated with placentation in dogs. Animal Reproduction, v. 17, n. 3, 2020-08-17. (13/09392-9, 15/17897-9)

VIDANE, A. S.; PINHEIRO, A. O.; CASALS, J. B.; PASSARELLI, D.; HAGE, M. C. F. N. S.; BUENO, R. S.; MARTINS, D. S.; AMBROSIO, C. E.. Transplantation of amniotic membrane-derived multipotent cells ameliorates and delays the progression of chronic kidney disease in cats. REPRODUCTION IN DOMESTIC ANIMALS, v. 52, n. 2, SI, p. 316-326, APR 2017. (13/09392-9)

MACHADO, LUCIANA C.; OLIVEIRA, VANESSA C.; PARAVENTI, MARIANA D.; CARDOSO, RAFAELA N. R.; MARTINS, DANIELE S.; AMBROSIO, CARLOS E.. Manutenção da Biodiversidade Brasileira através de bancos de germoplasma. Pesquisa Veterinária Brasileira, v. 36, n. 1, p. 62-66, JAN 2016. (13/09392-9)

GODOY PIERI, NAIRA CAROLINE; DE SOUZA, ALINE FERNANDA; BOTIGELLI, RAMON CESAR; MACHADO, LUCAS SIMOES; AMBROSIO, CARLOS EDUARDO; MARTINS, DANIELE DOS SANTOS; CESAR DE ANDRADE, ANDRE FURUGEN; MEIRELLES, FLAVIO VIEIRA; HYTTEL, POUL; BRESSAN, FABIANA FERNANDES. Stem cells on regenerative and reproductive science in domestic animals. VETERINARY RESEARCH COMMUNICATIONS, v. 43, n. 1, p. 7-16, FEB 2018. (13/08135-2, 13/09392-9, 12/01060-4, 15/26818-5)

DE ABREU, DILAYLA K.; MONTEIRO, JANAINA M.; SOUZA, CAROLINA C.; KARAM, RAFAEL G.; FERNANDES, RENATA A.; LESSA, THAIS B.; FAGLIARI, JOSE J.; MIGLINO, MARIA ANGELICA; AMBROSIO, CARLOS EDUARDO. Immunophenotyping lymphocyte and acute phase proteins in canine X-linked muscular dystrophy. Anais da Academia Brasileira de Ciências, v. 90, n. 3, p. 14-pg., 2018-07-01. (07/51222-2, 09/02958-1, 13/09392-9, 11/13030-0)

CASALS, JULIANA BARBOSA; GODOY PIERI, NAIRA CAROLINE; SANTOS ROBALLO, KELLY CRISTINE; BRESSAN, FABIANA FERNANDES; FAVARON, PHELIPE OLIVEIRA; MARTINS, DANIELE DOS SANTOS; AMBROSIO, CARLOS EDUARDO. Pluripotent stem cells proliferation is associated with placentation in dogs. ANIMAL REPRODUCTION, v. 17, n. 3, p. 10-pg., 2020-01-01. (15/17897-9, 13/09392-9)

CARDOSO, M. T.; PINHEIRO, A. O.; VIDANE, A. S.; CASALS, J. B.; DE OLIVEIRA, V. C.; GONCALVES, N. J. N.; MARTINS, D. S.; AMBROSIO, C. E.. Characterization of teratogenic potential and gene expression in canine and feline amniotic membrane-derived stem cells. REPRODUCTION IN DOMESTIC ANIMALS, v. 52, p. 7-pg., 2017-04-01. (13/09392-9, 13/23850-0)