Induced reprogramming of bovine somatic cells into induced pluripotent cells (IPS)...
| Full text | |
| Author(s): |
Godoy Pieri, Naira Caroline
[1]
;
de Souza, Aline Fernanda
[2]
;
Botigelli, Ramon Cesar
[3]
;
Machado, Lucas Simoes
[4]
;
Ambrosio, Carlos Eduardo
[2]
;
Martins, Daniele dos Santos
[2]
;
Cesar de Andrade, Andre Furugen
[1]
;
Meirelles, Flavio Vieira
[2]
;
Hyttel, Poul
[5]
;
Bressan, Fabiana Fernandes
[2]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Vet Med, Fac Anim Sci & Food Engn, Pirassununga - Brazil
[3] Sao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP - Brazil
[4] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo - Brazil
[5] Univ Copenhagen, Dept Vet & Anim Sci, Copenhagen - Denmark
Total Affiliations: 5
|
| Document type: | Review article |
| Source: | VETERINARY RESEARCH COMMUNICATIONS; v. 43, n. 1, p. 7-16, FEB 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
Stem cells are undifferentiated and self-renewable cells that present new possibilities for both regenerative medicine and the understanding of early mammalian development. Adult multipotent stem cells are already widely used worldwide in human and veterinary medicine, and their therapeutic signalling, particularly with respect to immunomodulation, and their trophic properties have been intensively studied. The derivation of embryonic stem cells (ESCs) from domestic species, however, has been challenging, and the poor results do not reflect the successes obtained in mouse and human experiments. More recently, the generation of induced pluripotent stem cells (iPSCs) via the forced expression of specific transcription factors has been demonstrated in domestic species and has introduced new potentials in regenerative medicine and reproductive science based upon the ability of these cells to differentiate into a variety of cells types in vitro. For example, iPSCs have been differentiated into primordial germ-like cells (PGC-like cells, PGCLs) and functional gametes in mice. The possibility of using iPSCs from domestic species for this purpose would contribute significantly to reproductive technologies, offering unprecedented opportunities to restore fertility, to preserve endangered species and to generate transgenic animals for biomedical applications. Therefore, this review aims to provide an updated overview of adult multipotent stem cells and to discuss new possibilities introduced by the generation of iPSCs in domestic animals, highlighting the possibility of generating gametes in vitro via PGCL induction. (AU) | |
| FAPESP's process: | 13/09392-9 - Production of induced pluripotent stem cells as model studies in rabbits |
| Grantee: | Carlos Eduardo Ambrósio |
| Support type: | Regular Research Grants |
| FAPESP's process: | 15/26818-5 - Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach |
| Grantee: | Fabiana Fernandes Bressan |
| Support type: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 12/01060-4 - Production of pluripotent stem cells as studied models in dogs |
| Grantee: | Carlos Eduardo Ambrósio |
| Support type: | Regular Research Grants |
| FAPESP's process: | 13/08135-2 - CTC - Center for Cell-Based Therapy |
| Grantee: | Dimas Tadeu Covas |
| Support type: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |