Multiuser equipment approved in grant 2013/13691-1 - for acquisition of AMNIS flow-sight - Millipore

• Grant number: 14/20809-1
• Support Opportunities: Multi-user Equipment Program
• Start date: December 01, 2014
• End date: November 30, 2016
• Field of knowledge: Biological Sciences - Pharmacology
• Principal Investigator: Regina Pekelmann Markus
• Grantee: Regina Pekelmann Markus
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/13691-1 - Immune-pineal axis: time-niology integrated to surveillance and defense, AP.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Processos Biológicos - Caracterização - Contagem células (inclui citômetros)
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The equipment is a confocal/facs allowing the separation of cells and the simultaneous evaluation of molecular markers and cell structures. In the project AMNIS will allow the analysis of phenotypic changes, gene expression, cell interaction, receptor expression and transduction pathways definition. In our project our goal is to understand the mechanisms involved in the activation and dysfunction of the immune-pineal axis. The Amnis is a quite versatile equipment that can be used by any researcher interested in understanding cellular function.The functional analysis is facilitated with AMNIS as simultaneously with cells separation we can evaluate the function of complex tissue. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MUXEL, SANDRA MARCIA; LARANJEIRA-SILVA, MARIA FERNANDA; ZAMPIERI, RICARDO ANDRADE; FLOETER-WINTER, LUCILE MARIA. Leishmania (Leishmania) amazonensis induces macrophage miR-294 and miR-721 expression and modulates infection by targeting NOS2 and L-arginine metabolism. SCIENTIFIC REPORTS, v. 7, MAR 9 2017. (14/50717-1, 10/52688-8, 14/20809-1, 12/15263-4)

MUXEL, SANDRA MARCIA; ACUNA, STEPHANIE MAIA; AOKI, JULIANA IDE; ZAMPIERI, RICARDO ANDRADE; FLOETER-WINTER, LUCILE MARIA. Toll-Like Receptor and miRNA-let-7e Expression Alter the Inflammatory Response in Leishmania amazonensis-Infected Macrophages. FRONTIERS IN IMMUNOLOGY, v. 9, NOV 29 2018. (16/03273-6, 17/23519-2, 14/20809-1, 14/50717-1, 16/19815-2)