Abstract
Intrauterine and early life nutritional deficiency favors metabolic and cardiovascular disorders such as hypertension and insulin resistance. In this context, insulinotropic amino acids may act as a complementary therapy. Currently our group investigates the effects of amino acids depletion in vitro on insulin secretion and action. So far, we have reported that the in vitro withdrawal of all amino acids reduces insulin secretion in a similar manner observed in the in vivo model of protein malnourished rodents. However, the individual role of different amino acids on the control of insulin secretion and action has not been established yet. Taurine is an amino acid synthesized from cysteine involved in several intracellular processes such as cellular volume and Ca2+ flux control. Among potential insulinotropic and insulinomimetic amino acids, taurine action on metabolic and endothelial homeostasis is detached in several studies. Although evidence point to the relevance of this amino acid, there are unanswered questions regarding the mechanisms by which its action occurs. Therefore, this proposal aims to investigate the intracellular mechanisms associated with insulinotropic, insulinomimetics and endothelial effects of taurine. To achieve these objectives, it will be employed in vitro cellular lines and animal protein malnutrition models to the study insulin, glucagon and somatostatin secretion and action focusing on dinamic oscilations of Ca2+, ATP, NAD(P)H and mitochondrial membrane potencial (Èm) in pancreatic islets, hepatocytes, miotubules and endothelial cells submitted to an in vitro protocol of protein restriction. (AU)
Scientific publications
(7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GARCIA-AREVALO, MARTA;
LORZA-GIL, ESTELA;
LEITE, NAYARA;
BRUNETTO, SERGIO;
BOSCHERO, ANTONIO CARLOS;
CARNEIRO, EVERARDO MAGALHAES.
The 17-Beta-Estradiol Improves Insulin Sensitivity in a Rapid Estrogen Receptor Alpha-Dependent Manner in an Animal Model of Malnourishment.
JOURNAL OF ENDOCRINOLOGY AND METABOLISM,
v. 9,
n. 5,
p. 133-146,
OCT 2019.
Web of Science Citations: 0.
GONCALVES, LUCIANA MATEUS;
VETTORAZZI, JEAN FRANCIESCO;
VANZELA, EMERIELLE CRISTINE;
FIGUEIREDO, MARIANA SARTO;
BATISTA, THIAGO MARTINS;
ZOPPI, CLAUDIO CESAR;
BOSCHERO, ANTONIO CARLOS;
CARNEIRO, EVERARDO MAGALHAES.
Amino acid restriction increases beta-cell death under challenging conditions.
Journal of Cellular Physiology,
v. 234,
n. 10,
p. 16679-16684,
OCT 2019.
Web of Science Citations: 0.
BORCK, PATRICIA C.;
BATISTA, THIAGO M.;
VETTORAZZI, JEAN F.;
SOARES, GABRIELA M.;
LUBACZEUSKI, CAMILA;
GUAN, DONGYIN;
BOSCHERO, ANTONIO C.;
VIEIRA, ELAINE;
LAZAR, MITCHELL A.;
CARNEIRO, EVERARDO M.
Nighttime light exposure enhances Rev-erb alpha-targeting microRNAs and contributes to hepatic steatosis.
METABOLISM-CLINICAL AND EXPERIMENTAL,
v. 85,
p. 250-258,
AUG 2018.
Web of Science Citations: 1.
BORCK, PATRICIA CRISTINE;
VETTORAZZI, JEAN FRANCIESCO;
SOUTO BRANCO, RENATO CHAVES;
BATISTA, THIAGO MARTINS;
SANTOS-SILVA, JUNIA CAROLINA;
NAKANISHI, VANESSA YUMI;
BOSCHERO, ANTONIO CARLOS;
RIBEIRO, ROSANE APARECIDA;
CARNEIRO, EVERARDO MAGALHAES.
Taurine supplementation induces long-term beneficial effects on glucose homeostasis in ob/ob mice.
Amino Acids,
v. 50,
n. 6,
p. 765-774,
JUN 2018.
Web of Science Citations: 5.
VETTORAZZI, JEAN FRANCIESCO;
KURAUTI, MIRIAN AYUMI;
SOARES, GABRIELA MOREIRA;
BORCK, PATRICIA CRISTINE;
FERREIRA, SANDRA MARA;
SOUTO BRANCO, RENATO CHAVES;
LIMA MICHELONE, LUCIANA DE SOUZA;
BOSCHERO, ANTONIO CARLOS;
COSTA JUNIOR, JOSE MARIA;
CARNEIRO, EVERARDO MAGALHAES.
Bile acid TUDCA improves insulin clearance by increasing the expression of insulin-degrading enzyme in the liver of obese mice.
SCIENTIFIC REPORTS,
v. 7,
NOV 1 2017.
Web of Science Citations: 8.
LUBACZEUSKI, CAMILA;
GONCALVES, LUCIANA MATEUS;
VETTORAZZI, JEAN FRANCIESCO;
KURAUTI, MIRIAN AYUMI;
SANTOS-SILVA, JUNIA CAROLINA;
BONFLEUR, MARIA LUCIA;
BOSCHERO, ANTONIO CARLOS;
COSTA-JUNIOR, JOSE MARIA;
CARNEIRO, EVERARDO MAGALHAES.
Vagotomy Reduces Insulin Clearance in Obese Mice Programmed by Low-Protein Diet in the Adolescence.
NEURAL PLASTICITY,
2017.
Web of Science Citations: 1.