In vivo and in vitro endothelial effects of taurine in protein restriction

• Grant number: 16/14461-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): January 01, 2017
• Effective date (End): December 31, 2021
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Ana Paula Couto Davel
• Grantee: Daniele Mendes Guizoni
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center, AP.CEPID

Abstract

Nutritional deficiency is a public health problem that affects mainly children of the de-veloping countries. Intrauterine and early life malnutrition favor metabolic and cardio-vascular disorders associated with endothelial dysfunction such as hypertension and Type 2 Diabetes. In this context, the insulinotropic, vasodilator and antioxidant amino acid taurine may act as a complementary therapy. However, the mechanisms by which taurine can be protective against endothelial dysfunction and hypertension still need to be elucidated. Therefore, this proposal aims to investigate the intracellular mechanisms associated with the endothelial effects of taurine following protein restriction. For this, we will use two methodological approaches: 1) aminoacid restriction in vitro through the exposure of endothelial cells to low amino acid content; 2) protein restriction in vivo by feeding post-weaned mice with an isocaloric low-protein diet (6% protein) for 90 days. In endothelial cells exposed to amino acid restriction and/or taurine, Nitric Oxide (NO) production and insulin signaling pathway will be evaluated. Mice fed low-protein diet will be concomitantly treated with taurine (2.5%). At the end of treatment, the systolic blood pressure will be evaluated by tail-cuff plethysmography and small mesenteric arteries and interlobular pancreatic arteries will be isolated for vascular function, protein expression and NO production. This study should contribute to elucidate potential therapeutic mechanisms of taurine in cardiovascular diseases associated with metabolic disorders. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BALTIERI, NATALI; GUIZONI, DANIELE M.; VICTORIO, JAMAIRA A.; DAVEL, ANA P.. Protective Role of Perivascular Adipose Tissue in Endothelial Dysfunction and Insulin-Induced Vasodilatation of Hypercholesterolemic LDL Receptor-Deficient Mice. FRONTIERS IN PHYSIOLOGY, v. 9, MAR 19 2018. (14/22506-6, 16/14461-8)

GUIZONI, DANIELE M.; FREITAS, ISRAELLE N.; VICTORIO, JAMAIRA A.; POSSEBOM, ISABELA R.; ARAUJO, THIAGO R.; CARNEIRO, EVERARDO M.; DAVEL, ANA P.. Taurine treatment reverses protein malnutrition-induced endothelial dysfunction of the pancreatic vasculature: The role of hydrogen sulfide. METABOLISM-CLINICAL AND EXPERIMENTAL, v. 116, MAR 2021. (14/01717-9, 13/07607-8, 16/14461-8)

GUIZONI, DANIELE M.; VETTORAZZI, JEAN F.; CARNEIRO, EVERARDO M.; DAVEL, ANA PAULA. Modulation of endothelium-derived nitric oxide production and activity by taurine and taurine-conjugated bile acids. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 94, p. 48-53, JAN 1 2020. (14/01717-9, 13/07607-8, 16/14461-8)