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In vivo and in vitro endothelial effects of taurine in protein restriction

Grant number: 16/14461-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2017
Effective date (End): September 30, 2021
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Ana Paula Couto Davel
Grantee:Daniele Mendes Guizoni
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:13/07607-8 - OCRC - Obesity and Comorbidities Research Center, AP.CEPID


Nutritional deficiency is a public health problem that affects mainly children of the de-veloping countries. Intrauterine and early life malnutrition favor metabolic and cardio-vascular disorders associated with endothelial dysfunction such as hypertension and type 2 diabetes. In this context, the insulinotropic, vasodilator and antioxidant amino acid taurine may act as a complementary therapy. However, the mechanisms by which taurine can be protective against endothelial dysfunction and hypertension still need to be elucidated. Therefore, this proposal aims to investigate the intracellular mechanisms associated with the endothelial effects of taurine following protein restriction. For this, we will use two methodological approaches: 1) aminoacid restriction in vitro through the exposure of endothelial cells to low amino acid content; 2) protein restriction in vivo by feeding post-weaned mice with an isocaloric low-protein diet (6% protein) for 90 days. In endothelial cells exposed to amino acid restriction and/or taurine, nitric oxide (NO) production and insulin signaling pathway will be evaluated. Mice fed low-protein diet will be concomitantly treated with taurine (2.5%). At the end of treatment, the systolic blood pressure will be evaluated by tail-cuff plethysmography and small mesenteric arteries and interlobular pancreatic arteries will be isolated for vascular function, protein expression and NO production. This study should contribute to elucidate potential therapeutic mechanisms of taurine in cardiovascular diseases associated with metabolic disorders. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GUIZONI, DANIELE M.; FREITAS, ISRAELLE N.; VICTORIO, JAMAIRA A.; POSSEBOM, ISABELA R.; ARAUJO, THIAGO R.; CARNEIRO, EVERARDO M.; DAVEL, ANA P. Taurine treatment reverses protein malnutrition-induced endothelial dysfunction of the pancreatic vasculature: The role of hydrogen sulfide. METABOLISM-CLINICAL AND EXPERIMENTAL, v. 116, MAR 2021. Web of Science Citations: 0.
GUIZONI, DANIELE M.; VETTORAZZI, JEAN F.; CARNEIRO, EVERARDO M.; DAVEL, ANA PAULA. Modulation of endothelium-derived nitric oxide production and activity by taurine and taurine-conjugated bile acids. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 94, p. 48-53, JAN 1 2020. Web of Science Citations: 1.
BALTIERI, NATALI; GUIZONI, DANIELE M.; VICTORIO, JAMAIRA A.; DAVEL, ANA P. Protective Role of Perivascular Adipose Tissue in Endothelial Dysfunction and Insulin-Induced Vasodilatation of Hypercholesterolemic LDL Receptor-Deficient Mice. FRONTIERS IN PHYSIOLOGY, v. 9, MAR 19 2018. Web of Science Citations: 6.

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