Tumor microenvironment in indolent non-Hodgkin lymphomas: role of tumoral microRNAs and inflammatory polymorphisms in clinical and pathological aspects

• Grant number: 14/09854-5
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2015
• End date: August 31, 2017
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: José Vassallo
• Grantee: José Vassallo
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Carmen Silvia Passos Lima ; Guilherme Rossi Assis de Mendonça ; Iscia Teresinha Lopes Cendes

Abstract

Indolent lymphomas (IL) represent a particular group among non-Hodgkin lymphomas (NHLs) that do not achieve complete response to therapy, which results in relapses. The inflammatory tumor microenvironment and tumoral microRNAs have been recently considered in progression of these tumors. This study aims to describe a detailed composition of the tumor microenvironment of mantle cell lymphoma and follicular lymphoma (MCL and FL, respectively) obtained from patients treated at the Blood Center at UNICAMP. In parallel, genetic polymorphisms in the immunity genes IL2, IL12A, IL10, TGFB1, TGFBR1, TGFBR2, IL17A and IL17F will be studied in each patient, which may interfere with the composition of the microenvironment cells and individual clinical course. MicroRNAs from tumor tissues will also be studied, in order to assess influence in the clinical outcome and in the composition of tumoral microenvironment. The inflammatory cells and the cytokines encoded by each gene here considered will be assessed by immunohistochemistry, and genotyping will be performed by the Openarray" system. The microRNAs will be studied by means of next-generation sequencing, performed by the Illumina® platform. Disease progression will be achieved by review of medical records. We hope that this study will contribute to characterize distinct groups of patients with MCL and FL, expanding the knowledge about their pathophysiology and enabling the development of more reliable prognostic markers for these diseases, in the context of personalized medicine. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ASSIS-MENDONCA, GUILHERME ROSSI; FATTORI, ANDRE; ROCHA, RAFAEL MALAGOLI; LOURENCO, GUSTAVO JACOB; DELAMAIN, MARCIA TORRESAN; NONOGAKI, SUELY; CORDEIRO DE LIMA, VLADMIR CLAUDIO; BRAGA COLLEONI, GISELE WALLY; DE SOUZA, CARMINO ANTONIO; SOARES, FERNANDO AUGUSTO; et al. Single nucleotide variants in immune-response genes and the tumor microenvironment composition predict progression of mantle cell lymphoma. BMC CANCER, v. 21, n. 1, MAR 1 2021. (14/09854-5)

ASSIS-MENDONCA, GUILHERME ROSSI; CAMPOS, LETICIA GOULART; DELAMAIN, MARCIA TORRESAN; DE BRITO, ANGELO BORSARELLI CARVALHO; FANELLI, MARCELLO FERRETI; SOARES, FERNANDO AUGUSTO; DE SOUZA, CARMINO ANTONIO; VASSALLO, JOSE; LIMA, CARMEN SILVIA PASSOS. Association of single nucleotide variants in VEGFA and KDR with the risk and angiogenic features of diffuse large B-cell lymphoma. Leukemia & Lymphoma, v. N/A, p. 13-pg., 2023-08-29. (15/15756-9, 14/09854-5)

GUILHERME ROSSI ASSIS-MENDONÇA; ANDRÉ HENRIQUE CREPALDI; MÁRCIA TORRESAN DELAMAIN; ADRIANA HELENA MOREIRA; FELIPE D'ALMEIDA COSTA; VLADMIR CLÁUDIO CORDEIRO DE LIMA; CÁRMINO ANTONIO DE SOUZA; FERNANDO AUGUSTO SOARES; JOSÉ VASSALLO. Characteristics of follicular and mantle cell lymphoma in Brazil: prognostic impact of clinical parameters and treatment conditions in two hospitals. Hematology, Transfusion and Cell Therapy, v. 40, n. 4, p. 343-353, 2018-10-00. (14/09854-5)