Rats facial nerve regeneration with bone marrow stem cells compared to dental pulp stem cells

• Grant number: 14/18764-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2015
• End date: March 31, 2016
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Heloísa Juliana Zabeu Rossi Costa
• Grantee: Heloísa Juliana Zabeu Rossi Costa
• Host Institution: Fundação Faculdade de Medicina da Universidade de São Paulo (FUNDFMUSP). São Paulo , SP, Brazil
• Associated researchers: Márcio Paulino Costa ; Ricardo Ferreira Bento

Abstract

Facial nerve palsy is a frequent and disabling condition and, if nerve section occurs, full functional recovery is seldom achieved. Bone marrow stem cells and dental pulp stem cells have proven to be effective on facial nerve regeneration, but there aren´t papers that compare the effects of these two cellular types for future clinical trials with this purpose. The aim of this study is to evaluate the functional and histological effects of bone marrow stem cells (BMSC) or dental pulp stem cells (DPSC) combined with polyglycolic acid tube (PGAt) in autografted rat facial nerves. After neurotmesis of the mandibular branch of the rat facial nerve, surgical repair will consist of nerve autografting contained in PGAt filled with basement membrane matrix (group A) with BMSC (group B) or DPSC (group C). Axon morphometrics and an objective compound muscle action potentials (CMAP) analysis will be conducted. Immunofluorescence assays will be carried out with Schwann cell marker S100 and anti-beta-galactosidase to label exogenous cells. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, LARISSA VILELA; BENTO, RICARDO FERREIRA; CRUZ, DAYANE B.; MARCHI, CLAUDIA; SALOMONE, RAQUEL; OITICICCA, JEANNE; COSTA, MARCIO PAULINO; HADDAD, LUCIANA A.; MINGRONI-NETTO, REGINA CELIA; ZABEU ROSSI COSTA, HELOISA JULIANA. Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate in vivo and Promote Facial Nerve Regeneration. CELL TRANSPLANTATION, v. 28, n. 1, p. 55-64, JAN 2019. (14/18764-0, 13/08028-1)