Effect of energy sources (starch, pectin or fat) and chromium supplementation, on high energy diet, on serum concentrations of glucose, insulin, and non-esterified fatty acids on lactating dairy cows

• Grant number: 14/13291-6
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2015
• End date: March 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: José Luiz Moraes Vasconcelos
• Grantee: José Luiz Moraes Vasconcelos
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Reinaldo Fernandes Cooke

Abstract

The objective of this study is to evaluate the influence of the energy source of the diet and chromium supplementation on serum concentrations of glucose, insulin, and non-esterified fatty acids (NEFA) on lactating cows. Forty multiparous lactating crossbred Holstein x Gir cows, within 80 ± 7 DIM will be blocked by milk yield and BCS, and randomly assigned to one of four treatments on d0 of the experiment: 1) diet formulated with corn to reach 160% of requirements for maintenance and lactation, without chromium supplementation (n=10); 2) the same of diet one with chromium supplementation (n=10; 2,5g of KemTRACETM Chromium Propionate 0,4% - Kemin Industries, Inc. - added in 97,5g of corn meal/animal/day) 3) diet formulated with citrus pulp (exp 1) or fat (exp 2) to reach 160% of requirements for maintenance and lactation, without chromium supplementation (n=10); 4) the same of diet three with chromium supplementation (n=10; 2,5g of KemTRACETM Chromium Propionate 0,4% - Kemin Industries, Inc. - added in 97,5g of corn meal/animal/day). Serum concentrations of glucose, insulin, and non-esterified fatty acids (NEFA) will be evaluated before feeding. Glucose Tolerance Test (n=3) will be performed on d-2, d100 and d200 to determine glucose clearance rate and insulin release. Blood samples will be taken at -15, 0 (before feeding), 10, 20, 30, 45, 60, 90 e 120 min after glucose infusion (0,5g dextrose/ kg de PV). Oocytes will picked-up (n=3) at d-4, d98 and d198 to evaluate oocyte and embryo production. All variables will be analyzed using the MIXED procedure of SAS. Serum concentrations of glucose, insulin, and non-esterified fatty acids will be analyzed as repeated measures. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEIVA, T.; COOKE, R. F.; BRANDAO, A. P.; BERTIN, R. D.; COLOMBO, E. A.; MIRANDA, V. F. B.; LOURENCO, L. A. C.; RODRIGUES, S. M. B.; VASCONCELOS, J. L. M.. Effects of supplemental calcium salts of palm oil and chromium-propionate on insulin sensitivity and productive and reproductive traits of mid- to late-lactating Holstein x Gir dairy cows consuming excessive energy. JOURNAL OF DAIRY SCIENCE, v. 101, n. 1, p. 491-504, JAN 2018. (14/14512-6, 14/13291-6)

LEIVA, T.; COOKE, R. F.; BRANDAO, A. P.; PARDELLI, U.; RODRIGUES, R. O.; CORRA, F. N.; VASCONCELOS, J. L. M.. Effects of concentrate type and chromium propionate on insulin sensitivity, productive and reproductive parameters of lactating dairy cows consuming excessive energy. ANIMAL, v. 11, n. 3, p. 436-444, MAR 2017. (14/13291-6, 15/21224-0, 14/14512-6)