Effect of energy density of the diet and chromium supplementation on serum concentrations of glucose, insulin, non-esterified fatty acids, triglycerides and IGF-1 on lactating dairy cows

• Grant number: 12/25390-3
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): July 01, 2013
• Effective date (End): February 28, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: José Luiz Moraes Vasconcelos
• Grantee: Tiago Leiva
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The objective of this study is to evaluate the influence of the energy density of the diet and chromium supplementation on the serum concentrations of glucose, insulin, non-esterified fatty acids (NEFA), triglycerides (TG) and insulin like growth factor 1 (IGF-1) lactating cows. Eighteen of crossbred Holstein x Gir cows, allocated in two pastures and receiving free-choice of corn silage and water, will be blocked by parturition (primiparous x multiparous) and milk yield, and randomly assigned to one of three treatments on d0 of the experiment: 1) Control: diet formulated to reach 100% of requirements for maintenance and lactation, without chromium supplementation (n=6); 2) GPSC: diet formulated to reach 100% and 160% of the requirements for lactation and maintenance, respectively, without chromium supplementation (n=6); 3) GPCC: diet formulated to meet 100% and 160% of the requirements for lactation and maintenance requirements, respectively, with chromium supplementation (n=6; 2,5g of KemTRACETM Chromium Propionate 0,4% - Kemin Industries, Inc. - added in 97,5g of corn meal/animal/day). Experimental diets will be fed twice daily. Treatments will be analyzed as contrast to indentify major contrast responses: Control versus GPSC to determine the effect of the energy density, and GPSC versus GPCC to determine the effect of chromium supplementation in high energy diets. Glucose Tolerance Test will be performed to determine the glucose clearance rate and insulin release. Oocytes will picked-up to determine their quality. All variables will be analyzed using the MIXED procedure of SAS (Inst., Inc., Cary, NC) and cow as experimental unit. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEIVA, T.; COOKE, R. F.; BRANDAO, A. P.; ABOIN, A. C.; RANCHES, J.; VASCONCELOS, J. L. M.. Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows. LIVESTOCK SCIENCE, v. 180, p. 121-128, OCT 2015. (12/24816-7, 12/25390-3)

LEIVA, T.; COOKE, R. F.; BRANDAO, A. P.; MARQUES, R. S.; VASCONCELOS, J. L. M.. Effects of rumen-protected choline supplementation on metabolic and performance responses of transition dairy cows. JOURNAL OF ANIMAL SCIENCE, v. 93, n. 4, p. 1896-1904, APR 2015. (12/25390-3)

LEIVA, T.; COOKE, R. F.; ABOIN, A. C.; DRAGO, F. L.; GENNARI, R.; VASCONCELOS, J. L. M.. Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters in nonlactating dairy cows. JOURNAL OF ANIMAL SCIENCE, v. 92, n. 2, p. 775-782, FEB 2014. (13/03939-6, 12/25390-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LEIVA, Tiago. Efeito da densidade nutricional da dieta e suplementação com cromo nas concentrações séricas de glicose, insulina e ácidos graxos não esterificados em vacas de leite lactantes. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.