Interference of drugs producing disturbances in the drug-receptor interactions in hypertension, hyperglycemia and neurotransmission emphasizing the modulator role of histamine

• Grant number: 14/01569-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2015
• End date: March 31, 2017
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Neide Hyppolito Jurkiewicz
• Grantee: Neide Hyppolito Jurkiewicz
• Host Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Aron Jurkiewicz

Abstract

The objective of this project is related to the mechanism of drug action from the pharmacological-chemical, molecular, and therapeutic points of view. The chosen drugs and methods might change the drug-receptor system on hypertension, hyperglycemia, and modulation by histamine. Subproject I: Study of cellular homeostasis of calcium in peripheral neurotransmission in cardiovascular and metabolic diseases. The objective is to: 1- To study the mechanisms the can lead to a sympathetic disfunction, analyzing mainly the release of catecholamines stimulated with angiotensin II. 2- To quantify the concentration of ATP in chromafin cells. 3- To study functional changes in isolated atrium, considering chronotropism and inotropism. 4- To study functional sympathetic changes, considering purines in isolate atrium considering chronotropism and inotropism. 5- Release of catecholamines stimulated by purinegic agonists. 6- To study cellular homeostasis of calcium. Subproject II: Modulatory effect of histamin in isolated rat testis capsule. Specific objectives: 1- Evaluate pharmacologically which are the histamine receptors involved in the modulation of sympathetic neurotransmission. 2- Analyze, through molecular biology, the expression of the level of transcripts for histamine receptors. 3- Verify if stored histamine is released from sympathetic peripheral terminals. 4- To study the interaction of postsynaptic responses induced by histamine. 5- To study the interaction of adrenergic and histaminergic auto receptors. 6- Evaluate the effect of histamine on maturation and of transit time of spermatozoids. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA BOMFIM, GUILHERME HENRIQUE; MENDEZ-LOPEZ, IAGO; CARLOS FERNANDEZ-MORALES, JOSE; FERNANDO PADIN, JUAN; JURKIEWICZ, ARON; JURKIEWICZ, NEIDE HYPPOLITO; GARCIA GARCIA, ANTONIO. Electrophysiological properties and augmented catecholamine release from chromaffin cells of WKY and SHR rats contributing to the hypertension development elicited by chronic EtOH consumption. European Journal of Pharmacology, v. 803, p. 65-77, MAY 15 2017. (14/01569-0)

BOMFIM, GUILHERME HENRIQUE SOUZA; MUSIAL, DIEGO CASTRO; MENDEZ-LOPEZ, IAGO; JURKIEWICZ, ARON; JURKIEWICZ, NEIDE HYPPOLITO; PADIN, JUAN FERNANDO; GARCIA, ANTONIO G.. Chronic resveratrol consumption prevents hypertension development altering electrophysiological currents and Ca2+ signaling in chromaffin cells from SHR rats. CELLULAR SIGNALLING, v. 76, DEC 2020. (14/01569-0)

SOUZA BOMFIM, GUILHERME HENRIQUE; MENDEZ-LOPEZ, IAGO; ALBERTO ARRANZ-TAGARRO, JUAN; MUSIAL, DIEGO CASTRO; FERRAZ CARBONEL, ADRIANA APARECIDA; FERNANDO PADIN, JUAN; GARCIA GARCIA, ANTONIO; FERRAZ JURKIEWICZ, ARON APARECIDA; JURKIEWICZ, NEIDE HYPPOLITO. Functional Upregulation of STIM-1/Orai-1-Mediated Store-Operated Ca2+ Entry Contributing to the Aortic Remodeling and Hypertension Development by Chronic EtOH Consumption. FASEB JOURNAL, v. 31, p. 1-pg., 2017-04-01. (14/01569-0)

SOUZA BOMFIM, GUILHERME HENRIQUE; MUSIAL, DIEGO CASTRO; MIRANDA-FERREIRA, REGIANE; NASCIMENTO, STHEFANI RODRIGUES; JURKIEWICZ, ARON; JURKIEWICZ, NEIDE HYPPOLITO; DE MOURA, ROBERTO SOARES. Antihypertensive effects of the Vitis vinifera grape skin (ACH09) extract consumption elicited by functional improvement of P1 (A(1)) and P2 (P2X1) purinergic receptors in diabetic and hypertensive rats. PHARMANUTRITION, v. 8, p. 12-pg., 2019-06-01. (14/01569-0)

SOUZA BOMFIM, GUILHERME HENRIQUE; MENDEZ-LOPEZ, IAGO; ALBERTO ARRANZ-TAGARRO, JUAN; FERRAZ CARBONEL, ADRIANA APARECIDA; ROMAN-CAMPOS, DANILO; FERNANDO PADIN, JUAN; GARCIA GARCIA, ANTONIO; JURKIEWICZ, ARON; JURKIEWICZ, NEIDE HYPPOLITO. Functional Upregulation of STIM-1/Orai-1-Mediated Store-Operated Ca2+ Contributing to the Hypertension Development Elicited by Chronic EtOH Consumption. CURRENT VASCULAR PHARMACOLOGY, v. 15, n. 3, p. 265-281, 2017. (14/01569-0)

SOUZA BOMFIM, GUILHERME HENRIQUE; MUSIAL, DIEGO CASTRO; ROCHA, KATIUCHA; JURKIEWICZ, ARON; JURKIEWICZ, NEIDE HYPPOLITO. Red wine but not alcohol consumption improves cardiovascular function and oxidative stress of the hypertensive-SHR and diabetic-STZ rats. CLINICAL AND EXPERIMENTAL HYPERTENSION, v. N/A, p. 12-pg., 2022-06-15. (14/01569-0)