Study of prostatic reactive stroma after bilateral orchiectomy and subsequent inhibition of aromatase activity by chrysin and inhibition of 5-alpha reductase activity by Finasteride

• Grant number: 14/26660-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2015
• End date: March 31, 2017
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Sebastião Roberto Taboga
• Grantee: Sebastião Roberto Taboga
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Associated researchers: Fernanda Cristina Alcantara dos Santos ; Luis Octávio Regasini ; Patricia Simone Leite Vilamaior ; Rejane Maira Góes

Abstract

The stromal reaction is a well known phenomenon in histopathology because it involves multiple proteins, cell-signaling and extracellular matrix events that provide a response to an epithelial tissue aggression, particularly neoplastic changes and inflammatory processes. Although it is well known the processes that lead to the formation of reactive stroma in prostate many questions about this phenomenon, so this project aims to identify, quantify and characterize stromal cells as well as key elements of the extracellular matrix remain associated with prostate reactive stroma in Mongolian gerbils subjected to different forms of androgen ablation and blockade of the enzymes 5±-reductase and aromatase. To achieve the objectives, experimental studies in vivo with adult and senile male gerbils will be employed. Our experimental design consists, in general, the assessment of the effects of chrysin (aromatase inhibitor) and finasteride (5-alpha-reductase inhibitor) applied in adult animals previously castrated and intact in specific times of 1, 4 and 72 weeks of experimental treatment. For evaluation of cell-cell and cell-extracellular matrix interactions features immunohistochemistry and immunofluorescence for specific markers of cells and extracellular matrix molecules will be employed. Analyzes will be made in light microscopy, confocal laser and electron transmission microscopies. The results expected from this research should provide a better understanding of the relationships between the cells involved in the genesis and dynamics of reactive stroma and correlate them with the extracellular environment that keeps this histopathological process. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FELISBINO, SERGIO LUIS; AZEVEDO SANCHES, BRUNO DOMINGOS; DELELLA, FLAVIA KARINA; SCARANO, WELLERSON RODRIGO; ALCANTARA DOS SANTOS, FERNANDA CRISTINA; LEITE VILAMAIOR, PATRICIA SIMONE; TABOGA, SEBASTIAO ROBERTO; JUSTULIN, LUIS ANTONIO. Prostate telocytes change their phenotype in response to castration or testosterone replacement. SCIENTIFIC REPORTS, v. 9, MAR 6 2019. (17/01063-7, 14/26660-0)

QUINTAR, AMADO A.; GONCALVES, BIANCA F.; TABOGA, SEBASTIAO R.; MALDONADO, CRISTINA A.. The mongolian gerbil (Meriones unguiculatus) as a model for inflammation-promoted prostate carcinogenesis. Cell Biology International, v. 41, n. 11, SI, p. 1234-1238, NOV 2017. (14/26660-0)

QUINTAR, AMADO A.; GONCALVES, BIANCA F.; TABOGA, SEBASTIAO R.; MALDONADO, CRISTINA A.. The mongolian gerbil (Meriones unguiculatus) as a model for inflammation-promoted prostate carcinogenesis. Cell Biology International, v. 41, n. 11, p. 5-pg., 2017-11-01. (14/26660-0)