The prostate is a gland commonly associated with the male urogenital system, but recently it has been described in females of several mammals, being a current target of experimental studies. This gland is regulated by steroid hormones and endocrine disruptions can affect its development and morphologic features. Studies regarding the exposure to endocrine disruptors, such as the xenoestrogen bisphenol A (BPA), presented relevant changes in prostatic morphophysiology. Moreover, exposure to BPA results in gene modulation and, consequently, in the expression of hormone receptors. Thus, the present study aims to analyze the expression of hormone receptors and intraprostatic hormonal modulation via converting enzymes in the prostate of aged female gerbils (Meriones unguiculatus) after exposure to BPA during gestation and lactation. For this study, the females will be divided into 3 experimental groups: control group (gavage with water); vehicle control group (gavage with oil); BPA group (50 µg/kg/daily). The samples will be submitted and analyzed under histological techniques, immunohistochemistry and immunofluorescence. The expression of androgen (AR), estrogen (ER± and ER²), progesterone (PR) and prolactin (PRL) receptors will be evaluated and associated to proliferation and cell death in the epithelium, lesions and stroma. Thus, this study will present mechanistic results linking the modulation of receptors to these cellular events. Also, serum hormone levels of testosterone and estrogen will be analyzed, in addition to the expression of hormone-converting enzymes, 5±-reductase and aromatase, in the female prostate. Thus, the present study will present results regarding the modulation of hormone receptors expression and in-tissue hormonal-converting enzymes in the female prostate after exposure to BPA.
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