Monitoring microbiological profile, endotoxins levels and potential inflammatory biomarkers for ventilator-associated pneumina in intensive care unit patients under invasive mechanical ventilation

• Grant number: 15/03696-1
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2015
• End date: June 30, 2017
• Field of knowledge: Health Sciences - Nursing
• Principal Investigator: Frederico Canato Martinho
• Grantee: Frederico Canato Martinho
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
• City of the host institution: São José dos Campos

Abstract

Pneumonia is the secondly most common hospital disease and mainly the major cause of mortally among the infections acquired under hospital environmental. The aspiration of microorganisms from inferior respiratory tract, followed by the proliferation and invasion of lung tissues consists the main and the most important via for the development of Ventilator-Associated Pneumonia (VAP). The knowledge of the microbial diversity and the identification of non-yet-cultivable bacteria in pneumonia seems to be important for the establishment of an effective therapy. Thus, The search for inflammatory markers in the diagnosis of Ventilator-Associated Pneumonia (VAP) have increased over the last years. Therefore, the aims of this clinical study are: 1) to study the microbial profile and diversity present in the oral cavity and in the bronchoalveolar lavage of patients hospitalized in ICU under VMI by Checkerboard DNA-DNA Hybridization technique; 2) to monitor the microbiota profile found in the oral cavity and in the bronchoalveolar of patients hospitalized in ICU under VMI by Checkerboard DNA-DNA Hybridization technique (12h, 48h e 96 h); 3) to investigate and monitor the levels of IL-1², TNF-±, IL-6 e IL-10 present in the bronchoalveolar lavage of patients hospitalized in ICU under VMI (12h, 48h e 96 h); 4) to investigate and monitor the levels of metaloproteinases (MMP1, MMP2 and MMP9) present in the bronchoalveolar lavage of patients hospitalized in ICU under VMI (12h, 48h e 96 h); 5) to investigate and monitor sTREM-1 (Soluble Triggering Receptor Expressed in Myeloid Cells-1) in the in the bronchoalveolar lavage of patients hospitalized in ICU under VMI; 6) Quantify endotoxins from Gram-negative bacteria species present in the oral cavity and in the bronchoalveolar lavage of patients hospitalized in ICU under VMI. Samples from the oral cavity (Crevicular fluid) using sterile/ apyrogenic paper points and bronchoal-alveolar lavage fluid by an oral tracheal suction catheter (mini-Bal). The microbial profile and diversity will be determined by molecular method - by Checkerboard DNA-DNA Hybridization technique. The levels of selected inflammatory biomarkers will be determined by ELISA assay (Enzyme-Linked Immunosorbent Assay). The levels of endotoxins will be quantified by the KQCL-test (LAL-assay). data will be tabulated and statistically analyzed by SPSS for Windows and package Statistic 9.0. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES, CINTHYA; MARTINHO, FREDERICO CANATO; BARBOSA, DECIO SABBATINI; ANTUNES, LEONARDO SANTOS; CORREA POVOA, HELVECIO CARDOSO; LEAL BALTUS, THIAGO HISSNAUER; MORELLI, NAYARA RAMPAZZO; VARGAS, HEBER ODEBRECHT; VARGAS NUNES, SANDRA ODEBRECHT; ANDERSON, GEORGE; et al. Increased Root Canal Endotoxin Levels are Associated with Chronic Apical Periodontitis, Increased Oxidative and Nitrosative Stress, Major Depression, Severity of Depression, and a Lowered Quality of Life. Molecular Neurobiology, v. 55, n. 4, p. 2814-2827, APR 2018. (15/03696-1)