Determining the role of cementocytes in dental cementum homeostasis

• Grant number: 15/06372-2
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2015
• End date: January 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Francisco Humberto Nociti Junior
• Grantee: Francisco Humberto Nociti Junior
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated researchers: Cristiane Ribeiro Salmon ; Kamila Rosamilia Kantovitz

Abstract

Cementocytes not only share morphological features with osteocytes, but also express several common biomakers. However, in contrast to osteocytes, there is a lack of evidence on the potential biological properties of cementocytes. Thus, we hypothesized that cementocytes play an important role in dental cementum homeostasis. In order to test our hypothesis, a comparative and systematic gene expression analysis will be performed using LCM/RNA-Seq on cementocytes in areas of active matrix apposition. In addition, morphological and ultrastructural changes in cementocytes will be investigated by transmission electron microscopy and confocal microscopy in samples labeled with fluorescein isothiocyanate. Lastly, based on our previous experience obtaining a cementocyte-like cell line from mouse (IDG-CM6), we aim at obtaining and characterizing cementocytes from human teeth in order to develop an in vitro model to propose functional assays. Based on our findings it is expected to define the role of cementocytes in dental cementum homeostasis and also identify potential biomolecules to be used in order to control dental cementum formation and regeneration. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMANDA BANDEIRA DE ALMEIDA; ELIS JANAÍNA LIRA DOS SANTOS; GABRIEL FLORES ABUNA; CRISTIANE SALMON RIBEIRO; MÁRCIO ZAFFALON CASATI; KARINA GONZALES SILVÉRIO RUIZ; FRANCISCO HUMBERTO NOCITI JUNIOR. Isolation and characterization of a human cementocyte-like cell line, HCY-23. Brazilian Oral Research, v. 33, 2019-08-15. (15/06372-2, 15/07232-0)

LIRA DOS SANTOS, ELIS J.; SALMON, CRISTIANE R.; CHAVEZ, MICHAEL B.; DE ALMEIDA, AMANDA B.; TAN, MICHELLE H.; CHU, EMILY Y.; SALLUM, ENILSON A.; CASATI, MARCIO Z.; RUIZ, KARINA G. S.; KANTOVITZ, KAMILA R.; et al. Cementocyte alterations associated with experimentally induced cellular cementum apposition in hyp mice. Journal of Periodontology, JUN 2021. (15/06372-2, 19/09435-6)

GIOVANI, PRISCILA A.; SALMON, CRISTIANE R.; MARTINS, LUCIAN; LEME, ADRIANA F. P.; PUPPIN-RONTANI, REGINA M.; MOFATTO, LUCIANA S.; NOCITI JR, FRANCISCO H.; KANTOVITZ, KAMILA R.. Membrane proteome characterization of periodontal ligament cell sets from deciduous and permanent teeth. Journal of Periodontology, v. 90, n. 7, p. 775-787, JUL 2019. (16/13786-0, 16/02942-1, 15/06372-2)

LIRA DOS SANTOS, ELIS J.; SALMON, CRISTIANE R.; CHAVEZ, MICHAEL B.; DE ALMEIDA, AMANDA B.; TAN, MICHELLE H.; CHU, EMILY Y.; SALLUM, ENILSON A.; CASATI, MARCIO Z.; RUIZ, KARINA G. S.; KANTOVITZ, KAMILA R.; et al. Cementocyte alterations associated with experimentally induced cellular cementum apposition in hyp mice. Journal of Periodontology, v. 92, n. 11, p. 116-127, NOV 2021. (15/06372-2, 19/09435-6)

DE ALMEIDA, AMANDA BANDEIRA; LIRA DOS SANTOS, ELIS JANAINA; ABUNA, GABRIEL FLORES; RIBEIRO, CRISTIANE SALMON; CASATI, MARCIO ZAFFALON; SILVERIO RUIZ, KARINA GONZALES; NOCITI JUNIOR, FRANCISCO HUMBERTO. Isolation and characterization of a human cementocyte-like cell line, HCY-23. BRAZILIAN ORAL RESEARCH, v. 33, p. 10-pg., 2019-01-01. (15/07232-0, 15/06372-2)