Assessment of arsenic's bioaccumulation and associated risks from fish consumption in Brazil

• Grant number: 15/08873-9
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2015
• End date: August 31, 2017
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Mario Henrique Gonzalez
• Grantee: Mario Henrique Gonzalez
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

Seafood market in Brazil have been expanded significantly in recent years, reaching an average consumption of 11.4 kg per capita in 2011 and with forecasting even further growth. Increasing search for for this food source in the country leads to a concern associated with fish quality, especially when dealing with toxic metals present in the waters and sediments of rivers. Fish, and other aquatic organisms, has the capacity to bioaccumulate metals in his organism, either by direct contact or feeding on other beings already contaminated. Arsenic is one of these contaminants and is present naturally in water and sediments, and also arising out human activities such as burning fossil fuels and pesticides. Most of the arsenic present in fish is in organic form (eg. Arsenobetaína) less toxic to humans, but there is a portion in its inorganic form (As (III) and (V)) and this must be given further attention due to their toxicity and carcinogenicity. This project has as main objectives the determination of the total arsenic content as well as the study of bioaccumulation of these contaminant, and assessment of risks to human health by calculating the estimated amount ingested by fish consumption in Brazil. Determinations will be made by an inductively coupled plasma mass spectrometry (ICP-MS) and samples digestion performed by microwave radiation in closed vessels. ICP-MS to be employed which allows working in the standard configuration and universal cell technology (UCT) enabling the instrument to operate in kinetic energy discrimination (KED), removing a large portion of the polyatomic interference. The estimate of arsenic intake allows assessing health risks by means of benchmarks set by international agencies such as Joint Food and Agriculture Organization of the United Nations / World Health Organization (FAO / WHO) Expert Committee on Food Additives (JECFA) which in 2011 recommended a intake value ranging from 2 to 7 µg kg-1 body weight per day, corresponding to the dose limit for a 0.5% increase in the incidence of lung cancer (BMDL05). The results of this project will be of paramount importance in the characterization of security levels in fish consumption in the region, and will be applied to them validation parameters of the methodology employing certified reference materials. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTANA, ANA PR; MORA-VARGAS, JORGE A.; GUIMARAES, TACIANA G. S.; AMARAL, CLARICE D. B.; OLIVEIRA, ANDREA; GONZALEZ, MARIO H.. Sustainable synthesis of natural deep eutectic solvents (NADES) by different methods. JOURNAL OF MOLECULAR LIQUIDS, v. 293, NOV 1 2019. (17/09520-8, 17/13230-5, 13/23886-4, 15/08873-9, 17/18531-3)

FERREIRA, SABRINA S.; BRITO, THOMAS A.; SANTANA, ANA P. R.; GUIMARAES, TACIANA G. S.; LAMARCA, RAFAELA S.; FERREIRA, KAREN C.; GOMES, PAULO C. F. LIMA; OLIVEIRA, ANDREA; AMARAL, CLARICE D. B.; GONZALEZ, MARIO H.. Greenness of procedures using NADES in the preparation of vegetal samples: Comparison of five green metrics. TALANTA OPEN, v. 6, p. 7-pg., 2022-12-01. (21/14581-1, 19/22113-8, 14/50945-4, 15/08873-9, 17/18531-3)

SANTANA, ANA P. R.; NASCIMENTO, PATRICIA DE ANDRADE; GUIMARAES, TACIANA G. S.; MENEZES, IOHANNA M. N. RIBEIRO; ANDRADE, DANIEL F.; OLIVEIRA, ANDREA; GONZALEZ, MARIO H.. (Re) thinking towards a sustainable analytical chemistry: Part I: Inorganic elemental sample treatment, and Part II: Alternative solvents and extraction techniques. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 152, p. 34-pg., 2022-04-08. (15/08873-9, 19/22113-8, 14/50945-4, 17/18531-3)

NASCIMENTO, PATRICIA A.; SCHULTZ, JULIANA; GONZALEZ, MARIO H.; OLIVEIRA, ANDREA. Simple GFAAS Method for Determination of Pb, As, and Cd in Cannabidiol Extracts Used for Therapeutic Purposes. Journal of the Brazilian Chemical Society, v. 31, n. 5, p. 894-903, MAY 2020. (13/23886-4, 17/09520-8, 17/13230-5, 17/18531-3, 15/08873-9)

OLIVEIRA, LUCIANO H. B.; FERREIRA, NATHALIA S.; OLIVEIRA, ANDREA; NOGUEIRA, ANA RITA A.; GONZALEZ, MARIO H.. Evaluation of Distribution and Bioaccumulation of Arsenic by ICP-MS in Tilapia (Oreochromis niloticus) Cultivated in Different Environments. Journal of the Brazilian Chemical Society, v. 28, n. 12, p. 2455-2463, DEC 2017. (16/05079-2, 15/08873-9)

MARIO H. GONZALEZ; CAROLINE S. DA SILVA; CLARICE D. B. AMARAL; SILMARA R. BIANCHI; LUCIANO H. B. DE OLIVEIRA; JÉSSICA S. COELHO; ANDREA OLIVEIRA; ANA RITA A. NOGUEIRA. Determination of Elemental Impurities in Acyclovir Ointment and Raw Materials Using Microwave Acid Digestion (MW-AD) and ICP-MS. Journal of the Brazilian Chemical Society, v. 28, n. 1, p. 98-105, 2017-01-00. (15/08873-9)

FERREIRA, NATHALIA S.; OLIVEIRA, LUCIANO H. B.; AGRELLI, VITOR; DE OLIVEIRA, ALINE F.; NOGUEIRA, ANA R. A.; OLIVEIRA, ANDREA; GONZALEZ, MARIO H.. Bioaccumulation and acute toxicity of As(III) and As(V) in Nile tilapia (Oreochromis niloticus). Chemosphere, v. 217, p. 349-354, FEB 2019. (17/18531-3, 16/05079-2, 16/24868-8, 15/08873-9)

SANTANA, ANA P. R.; ANDRADE, DANIEL F.; GUIMARAES, TACIANA G. S.; AMARAL, CLARICE D. B.; OLIVEIRA, ANDREA; GONZALEZ, MARIO H.. Synthesis of natural deep eutectic solvents using a mixture design for extraction of animal and plant samples prior to ICP-MS analysis. Talanta, v. 216, AUG 15 2020. (17/18531-3, 15/08873-9, 16/17304-0)

RIBEIRO MENEZES, IOHANNA M. N.; NASCIMENTO, PATRICIA DE A.; GONZALEZ, MARIO H.; OLIVEIRA, ANDREA. Simple and Robust GFAAS Methods for Determination of As, Cd, and Pb in Hemp Products Using Different Sample Preparation Strategies. FOOD ANALYTICAL METHODS, v. 14, n. 5, JAN 2021. (15/08873-9, 17/18531-3, 19/22113-8)

MOREIRA, LUANA S.; SANTANA, ANA P. R.; COSTA, FLORIATAN S.; VERA, JOSIANE B.; GONZALEZ, MARIO H.; DA SILVA, ERIK G. P.; AMARAL, CLARICE D. B.. Enhanced extraction of arsenic and cadmium from environmental samples using a natural deep eutectic solvent and determination by inductively coupled plasma mass spectrometry. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, v. 102, n. 18, p. 12-pg., 2020-09-29. (14/50945-4, 19/22113-8, 17/18531-3, 15/08873-9)