A new anatomical technique to the surgical teaching in dogs corpses: use of the ethanol and the chloride sodium

• Grant number: 15/08259-9
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2015
• End date: July 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Fabrício Singaretti de Oliveira
• Grantee: Fabrício Singaretti de Oliveira
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Andrigo Barboza de Nardi ; Bruno Watanabe Minto ; Marcia Rita Fernandes Machado

Abstract

In order to avoid tissue deterioration, anatomical pieces are fixed. Fixation technique is extremely important, since it keeps tissues strong, insoluble and protected. Formaldehyde is the most used fixing material and preservative, and, although it is still very useful at anatomy labs, it's hazardous and also brings lots of environmental risks. Other agents, as ethanol, have been used as good fixing agents for birds muscle, as has the Sodium chloride been testes as preservative for anatomical pieces. Besides, it is getting more and more important to have a lot of criteria about animal utilization in research and teaching activities and, consequently, look out for alternative methods for these uses, in a way that will not cause scientific or academic losses. In this way, this research aims to evaluate the possibility of a new anatomical technique that uses ethylic alcohol on fixation and sodium chloride aqueous solution on conservation of corpses, to quantify tissue resistance changes of those corpses during fixation and conservation through four months and identify and quantify, microbiologically, the main species present. Thus, dogs corpses will be submitted to different periods of fixation on ethylic alcohol and conservation on sodium chloride aqueous solution. Skin, jejunum, extern jugular vein and extern carotid common artery will be biomechanically tested and the fixation/conservation solutions tested for microbiology. First results show a great potential in using these substances for fixing and keeping the anatomical pieces for four months, in a way that they keep flexibility and viscera aspect similar to a fresh corpse. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, NATALIA; CARDOZO, MARITA VEDOVELLI; DE SA ROCHA, THIAGO ANDRE SALVITTI; ZERO, RAPHAEL CHIARELO; DE AVILA, FERNANDO ANTONIO; DE OLIVEIRA, FABRICIO SINGARETTI. Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution. SEMINA-CIENCIAS AGRARIAS, v. 40, n. 6, 2, p. 3099-3105, NOV-DEC 2019. (15/08259-9, 15/08826-0)

PEREIRA, NATALIA; CARDOZO, MARITA VEDOVELLI; DE SA ROCHA, THIAGO ANDRE SALVITTI; ZERO, RAPHAEL CHIARELO; DE AVILA, FERNANDO ANTONIO; DE OLIVEIRA, FABRICIO SINGARETTI. Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution. SEMINA-CIENCIAS AGRARIAS, v. 40, n. 6, p. 7-pg., 2019-11-01. (15/08826-0, 15/08259-9)

OLIVEIRA, FABRICIO SINGARETTI; ZERO, RAPHAEL CHIARELO; ROCHA, THIAGO ANDRE SALVITTI DE SA; MINTO, BRUNO WATANABE; MORAES, PAOLA CASTRO; DIAS, LUIS GUSTAVO GOSUEN GONCALVES; DE NARDI, ANDRIGO BARBOZA. Chemically Prepared Cat Cadavers in Teaching of Surgical Technique Evaluation by Students of a Veterinary Medicine Course. FASEB JOURNAL, v. 32, n. 1, p. 2-pg., 2018-04-01. (15/08259-9)