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Suture analysis and vein traction test in dogs fixed on alcohol/glycerin and conserved on saline solution

Grant number: 15/07939-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2015
Effective date (End): May 31, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:Fabrício Singaretti de Oliveira
Grantee:Marina Emanoella Seruti Pelógia
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil


In order to avoid tissue deterioration, anatomical pieces are fixed. Fixation technique is extremely important, since it keeps tissues strong, insoluble and protected. Formaldehyde is the most used fixing material and preservative, and, although it is still very useful at anatomy labs, it's hazardous and also brings lots of environmental risks. Other agents, as ethanol, have been used as good fixing agents for birds muscle, as has the Sodium chloride been testes as preservative for anatomical pieces. Besides, it is getting more and more important to have a lot of criteria about animal utilization in research and teaching activities and, consequently, look out for alternative methods for these uses, in a way that will not cause scientific or academic losses. In this way, this research aims to quantify tissue resistance changes on the extern jugular vein of dogs corpses during different times of fixation on ethylic alcohol and conservation on sodium chloride aqueous solution and to analyze the venous suture quality by using a surgical microscopy during the process of fixation/conservation. First results show a great potential in using these substances for fixing and keeping the anatomical pieces for four months, in a way that they keep flexibility and viscera aspect similar to a fresh corpse.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PELOGIA, MARINA E. S.; CERQUEIRA, EDUARDO S. F.; SILVEIRA, CAMILA P. B.; ROLIM, GLAUCO S.; FECHIS, ALISSON D. S.; ROCHA, THIAGO A. S. S.; LAUS, JOSE L.; OLIVEIRA, FABRICIO S. Suture and venous traction test analysis in dogs fixed in alcohol and preserved in saline solutions. Pesquisa Veterinária Brasileira, v. 39, n. 9, p. 1834-1837, SEP 2018. Web of Science Citations: 0.
MARINA E.S. PELÓGIA; EDUARDO S.F. CERQUEIRA; CAMILA P.B. SILVEIRA; GLAUCO S. ROLIM; ALISSON D.S. FECHIS; THIAGO A.S.S. ROCHA; JOSÉ L. LAUS; FABRÍCIO S. OLIVEIRA. Suture and venous traction test analysis in dogs fixed in alcohol and preserved in saline solution. Pesquisa Veterinária Brasileira, v. 38, n. 9, p. -, Set. 2018.

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