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Biomechanical analysis of the jejunum of dogs' corpses submitted to short-time formaldehyde fixation and conservation in 30% sodium chloride aqueous solution

Grant number: 15/22122-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2016
Effective date (End): May 31, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:Fabrício Singaretti de Oliveira
Grantee:Rafael Solano Dias
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

In order to avoid tissue deterioration, anatomical pieces are fixed. Formaldehyde is the most used fixing material and preservative, and, although it is still very useful at anatomy labs, it's hazardous and also brings lots of health risks. Other agents, as the Sodium chloride, have been tested as preservative for anatomical pieces. Besides, it is getting more and more important to have a lot of criteria about animal utilization in research and teaching activities and, consequently, look out for alternative methods for these uses, in a way that will not cause scientific or academic losses. The aim of this study is to check the biomechanical effect inflicted on the jejunum of corpses of dogs that went through a short-time formaldehyde fixation and conservation on a 30% sodium chloride aqueous solution. Changes on jejune resistance will be analyzed, measured in Newtons or Kgf, during the biomechanical traction test, on the moments 1, 3, 5 and 7 days of fixation on formaldehyde and 30, 60, 90 and 120 days of conservation on a 30% aqueous solution sodium chloride. It will be achieved if the short-time fixation (7 days), with posterior conservation on aqueous solution sodium chloride, changes the intestine resistance. The comparison with the control group (fresh corpses) will define if there is data similarity, recommending or not those corpses for surgical technique teaching in intestine tissue.

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