Assessment of early exposure to high-fat diet on protein involved in neurogenesis: studies, in vivo in hippocampus of adult mice and in vitro in neuroblast

• Grant number: 14/26146-4
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2015 - August 31, 2017
• Field of knowledge: Health Sciences - Nutrition - Malnutrition and Physiological Development
• Principal Investigator: Cristiano Mendes da Silva
• Grantee: Cristiano Mendes da Silva
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated researchers: Fernando Moreira Simabuco

Abstract

Maternal high-fat diet (HFD) during periods of pregnancy and lactation promotes neurological injuries to offspring, such as: 1) damage on hippocampus-dependent processes learning and memory; 2) changes in neurotransmitter systems (serotoninergic system, e.g.); and 3) change in the signaling pathway for neuronal proliferation/differentiation. These possible effects morphological and functional can be associated with changes in the neurogenic molecular mechanisms. In addition, Notch signaling pathway plays a key role, since it negatively modulates neuronal differentiation. Activation of the Notch pathway leads to up-regulation of the target gene Hes5, which inhibit the pro-neural gene Mash1. Thus, one exacerbated answer of this signaling pathway may possibly impair the hippocampal neurogenesis. Besides modulate the Notch signaling pathway, early exposure HFD can also change the development of neurotransmitter system of the serotonin (5-hydroxytryptamine, 5-HT), that plays a pro-neurogenic role. Thus, we believe that mice offspring's brains early exposure to maternal HFD, as well as exposure in vitro of neuroblasts (immature neurons) to saturated fatty acids may possibly promote changes in the molecular events that modulate neurogenic process. Therefore, the present research will evaluate the Notch signaling pathway and the development serotonergic system: in hippocampus of the adult mice offspring from dams exposure to HFD during pregnancy and lactation; and in neuroblast in vitro (Neuro-2a cells) exposure to saturated fatty acids. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIAS, CLARISSA TAVARES; CURI, HAIDAR TAFNER; PAYOLLA, TANYARA BALIANI; LEMES, SIMONE FERREIRA; BETIM PAVAN, ISADORA CAROLINA; TORSONI, MARCIO ALBERTO; SIMABUCO, FERNANDO MOREIRA; LAMBERTUCCI, RAFAEL HERLING; DA SILVA, CRISTIANO MENDES. Maternal high-fat diet stimulates proinflammatory pathway and increases the expression of Tryptophan Hydroxylase 2 (TPH2) and brain-derived neurotrophic factor (BDNF) in adolescent mice hippocampus. NEUROCHEMISTRY INTERNATIONAL, v. 139, OCT 2020. (18/14818-9, 15/08441-1, 14/26146-4)

CURI, HAIDAR TAFNER; DIAS, CLARISSA TAVARES; MONTEIRO DA LUZ CAMARGO, MARIA LAURA; GOMEZ, PAULA DOS SANTOS; PEREIRA GOMES, MOISES FELIPE; ARAUJO BESERRA-FILHO, JOSE IVO; MEDEIROS, ALESSANDRA; RIBEIRO, ALESSANDRA MUSSI; SIMABUCO, FERNANDO MOREIRA; LAMBERTUCCI, RAFAEL HERLING; et al. Maternal high-fat diet increases anhedonic behavior and modulates hippocampal Mash1 and BDNF expression in adult offspring. Neuroscience Letters, v. 764, NOV 1 2021. (15/08441-1, 18/10380-9, 14/26146-4)