Cytotoxic, genotoxic and mutagenic assessment of the Crataegus oxyacantha fruit extract: in vitro, in vivo, histological and ultra-structural analysis

• Grant number: 14/26882-2
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2015
• End date: November 30, 2017
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Edson Luis Maistro
• Grantee: Edson Luis Maistro
• Host Institution: Faculdade de Filosofia e Ciências (FFC). Universidade Estadual Paulista (UNESP). Campus de Marília. Marília , SP, Brazil
• Associated researchers: Maria Izabel Souza Camargo

Abstract

The industrial and technological development in general has the inevitable effect of human exposure to an increasing number of agents that, on the one hand, increase you comfort and wealth, but, on the other hand, increase a risk to their health. So, it is essential to establish standards for the use of these agents, based on studies identifying those with genetic toxicity effects, and may highlight the mutagenic, teratogenic or carcinogenic potential. Among these agents are the plants with medicinal properties. Its use dates back centuries and the popularity of them is connected to the easy access, therapeutic efficacy, relative low cost and the idea of complete absence of toxic effects. However, recently the safety of some of these herbal compounds have been questioned, due to reports of toxicity and fatalities. Crataegus oxyacantha is a native plant from Europe, however, due to its medicinal potential, it has spread to several countries, including Brazil. Its fruits have been used as diuretics in the treatment of dyspnea and renal calculus. There are also studies showing their sedative, ancyolitic, hypolipidemic, hypocholesterolemic and anti-atherosclerotic effects. Aditionaly, several studies have produced evidence about its benefit effects on heart failure. The fruits of Crataegus has also been used as foodstuff by humans, like canned fruit, jam, jelly, drink and wine. Based on the fact that C. oxyacantha extracts have been traditionally used for medicinal purposes by humans and that there are no studies involving the investigation of the cytotoxic and genotoxic potential of its fruits extract, regarding cell and genome integrity in mammalian cells, this project was designed to evaluate the cytotoxic, genotoxic mutagenic and clastogenic/aneugenic potential of this extract in vitro (using limphocytes, HepG2 human cells; and the AMES test in Salmonella) and in vivo (in different somatic and germ cells of mice) by the Comet assay and micronucleus test; Furthermore, will be analyzed the cytotoxic effects of the extract in liver cells of mice through the use of histological and ultrastructural techniques. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE QUADROS, ANA PAULA OLIVEIRA; CHRISTOFOLETTI MAZZEO, DANIA ELISA; MARIN-MORALES, MARIA APARECIDA; PERAZZO, FABIO FERREIRA; PIRES ROSA, PAULO CESAR; MAISTRO, EDSON LUIS. Fruit extract of the medicinal plant Crataegus oxyacantha exerts genotoxic and mutagenic effects in cultured cells. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 80, n. 3, p. 161-170, 2017. (14/26882-2)

ORLANDO, JULIANA BOTINHON; SILVA, BRIAN OGUSHI; PIRES-CUNHA, CAMILA LEHNHARDT; HIRUMA-LIMA, CLELIA AKIKO; DE MASCARENHAS GAIVAO, ISABEL O'NEILL; MAISTRO, EDSON LUIS. Genotoxic effects induced by beta-myrcene following metabolism by liver HepG2/C3A human cells. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 82, n. 3, p. 176-185, FEB 1 2019. (14/26882-2)

MAISTRO, EDSON LUIS; TERRAZZAS, PETERSON MENEZES; PERAZZO, FABIO FERREIRA; GAIVAO, ISABEL O'NEILL DE MASCARENHAS; SAWAYA, ALEXANDRA CHRISTINIE HELENA FRANKLAND; ROSA, PAULO CESAR PIRES. Salix alba (white willow) medicinal plant presents genotoxic effects in human cultured leukocytes. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 82, n. 23-24, JAN 2020. (14/26882-2)

SOUZA, ANA C. S.; SILVA, LAIS K.; QUEIROZ, THAIS B.; MARQUES, EDUARDO S.; HIRUMA-LIMA, CLELIA A.; GAIVAO, ISABEL O. M.; MAISTRO, EDSON L.. Citral presents cytotoxic and genotoxic effects in human cultured cells. DRUG AND CHEMICAL TOXICOLOGY, v. 43, n. 4, MAR 2019. (14/26882-2)

DE QUEIROZ, T. B.; DUPEYRON, D.; CARVALHO, J. C. T.; GAIVAO, I. O. M.; MAISTRO, E. L.. Anthocyanins-loaded Eudragit (R) L100 nanoparticles: in vitro cytotoxic and genotoxic analysis. Genetics and Molecular Research, v. 16, n. 4, NOV 2 2017. (14/26882-2)

FRODER, J. G.; DUPEYRON, D.; CARVALHO, J. C. T.; MAISTRO, E. L.. In vitro study of the cytotoxic and genotoxic effects of indomethacin-loaded Eudragit (R) L 100 nanocapsules. Genetics and Molecular Research, v. 15, n. 3, 2016. (14/26882-2)

QUEIROZ, T. B.; SANTOS, G. F.; VENTURA, S. C.; HIRUMA-LIMA, C. A.; GAIVAO, I. O. M.; MAISTRO, E. L.. Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2). Genetics and Molecular Research, v. 16, n. 3, OCT 3 2017. (14/26882-2)