CELLULAR STRESS AND ITS RELATION WITH THE KINECTICS OF IN VITRO PRODUCED BOVINE EMBRYOS

• Grant number: 15/03381-0
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2015
• End date: February 28, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marcella Pecora Milazzotto
• Grantee: Marcella Pecora Milazzotto
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

In vitro production of bovine embryos is still considered a low-efficiency biotechnology, once the technique has, on average, lower pregnancy rates when compared to in vivo. Although the in vitro culture system tries to mimic what occurs in vivo, embryos end up being exposed to various stress factors that they would usually do not suffer inside the reproductive tract. In addition to intrinsic factors such as the production of energy to sustain the developmental events, environmental stress factors caused by a non-optimized culture system may also impair embryonic development. For example, if oxidative homeostasis is not maintained, it may lead to ATP production and protein synthesis collapse. For the optimization of the culture processes, it is necessary to know through what types of stressful conditions these embryos are undergoing on the in vitro system and how these embryos respond to these stress conditions. For this reason, we believe that it is extremely important to investigate molecular mechanisms in order to establish a relationship between embryo competence and the cellular stressors. For this purpose, the combination of molecular, biochemical and morphological evaluation techniques may provide biomarkers of embryi response to stress in order to allow us to develop alternative strategies for modulation and empirical reformulation of culture methods aiming at the optimization and selection of embryos with best quality and viability. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LIMA, CAMILA BRUNA; FERREIRA, CHRISTINA RAMIRES; MILAZZOTTO, MARCELLA PECORA; SOBREIRA, TIAGO JOSE P.; VIREQUE, ALESSANDRA APARECIDA; GRAHAM COOKS, R.. Comprehensive lipid profiling of early stage oocytes and embryos by MRM profiling. Journal of Mass Spectrometry, v. 53, n. 12, p. 1247-1252, DEC 2018. (15/03381-0)

ISPADA, JESSICA; DA FONSECA JUNIOR, ALDCEJAM MARTINS; RAMOS SANTOS, OTAVIO LUIZ; DE LIMA, CAMILA BRUNA; DOS SANTOS, ERIKA CRISTINA; DA SILVA, VINICIUS LOURENCO; ALMEIDA, FERNANDA NASCIMENTO; LEITE, SAUL DE CASTRO; ROSS, PABLO JUAN; MILAZZOTTO, MARCELLA PECORA. Metabolism-driven post-translational modifications of H3K9 in early bovine embryos. Reproduction, v. 162, n. 3, p. 181-191, SEP 2021. (17/18384-0, 18/11668-6, 15/03381-0)

ALVES, GLAUCIA PEREIRA; BERTUCCEZ CORDEIRO, FERNANDA; DE LIMA, CAMILA BRUNA; ANNES, KELLY; DOS SANTOS, ERIKA CRISTINA; ISPADA, JESSICA; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; NICHI, MARCILIO; MILAZZOTTO, MARCELLA PECORA. Follicular environment as a predictive tool for embryo development and kinetics in cattle. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 31, n. 3, p. 451-461, FEB 2019. (12/10351-2, 12/50533-2, 15/03381-0)

DE LIMA, CAMILA BRUNA; DOS SANTOS, ERIKA CRISTINA; ISPADA, JESSICA; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; DIAS DOS SANTOS, CHARLES MORPHY; MILAZZOTTO, MARCELLA PECORA. The dynamics between in vitro culture and metabolism: embryonic adaptation to environmental changes. SCIENTIFIC REPORTS, v. 10, n. 1, SEP 24 2020. (12/50533-2, 17/18384-0, 15/03381-0, 17/11768-8)

ISPADA, JESSICA; DE LIMA, CAMILA BRUNA; SIRARD, MARC-ANDRE; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; ANNES, KELLY; MILAZZOTTO, MARCELLA PECORA. Genome-wide screening of DNA methylation in bovine blastocysts with different kinetics of development. EPIGENETICS & CHROMATIN, v. 11, JAN 8 2018. (15/03381-0)

DOS SANTOS, E. C.; VARCHETTA, R.; DE LIMA, C. B.; ISPADA, J.; MARTINHO, H. S.; FONTES, P. K.; NOGUEIRA, M. F. G.; GASPARRINI, B.; MILAZZOTTO, M. P.. The effects of crocetin supplementation on the blastocyst outcome, transcriptomic and metabolic profile of in vitro produced bovine embryos. Theriogenology, v. 123, p. 30-36, JAN 1 2019. (15/03381-0)

ISPADA, JESSICA; MILAZZOTTO, MARCELLA PECORA. ilencing mark H3K27me3 is differently reprogrammed in bovine embryos with distinct kinetics of developmen. REPRODUCTION IN DOMESTIC ANIMALS, v. 57, n. 3, DEC 2021. (18/11668-6, 15/03381-0, 19/25982-7)

LEITE, ROBERTA FERREIRA; ANNES, KELLY; ISPADA, JESSICA; DE LIMA, CAMILA BRUNA; DOS SANTOS, ERIKA CRISTINA; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; MILAZZOTTO, MARCELLA PECORA. Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on In Vitro Produced Embryos (vol 2017, 1502489, 2017). OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2018, p. 1-pg., 2018-01-01. (15/03381-0)

ISPADA, JESSICA; DA FONSECA JUNIOR, ALDCEJAM MARTINS; DE LIMA, CAMILA BRUNA; DOS SANTOS, ERIKA CRISTINA; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; DA SILVA, VINICIUS LOURENCO; ALMEIDA, FERNANDA NASCIMENTO; LEITE, SAUL DE CASTRO; CHITWOOD, JAMES LEE; et al. Tricarboxylic Acid Cycle Metabolites as Mediators of DNA Methylation Reprogramming in Bovine Preimplantation Embryos. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 18, p. 16-pg., 2020-09-01. (12/50533-2, 18/11668-6, 17/18384-0, 15/03381-0)