Differential profile of gene expression in cumulus cells of infertile women with and without endometriosis who underwent ovarian stimulation

• Grant number: 14/05878-7
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2015
• End date: October 31, 2017
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Paula Andrea de Albuquerque Salles Navarro
• Grantee: Paula Andrea de Albuquerque Salles Navarro
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Caroline Mantovani da Luz ; Daniel Blasioli Dentillo ; Juliana Meola Lovato ; Liliane Fabio Isidoro da Silva ; Rui Alberto Ferriani ; Wilson Araújo da Silva Junior

Abstract

A possible deleterious effect of endometriosis on oocyte quality (OQ) is questioned. Human oocytes are extremely rare and their use in invasive studies, make their use in assisted reproduction procedures impossible. Thus, the identification of non-invasive biomarkers capable of predicting the OQ, as cumulus cells (CCs), is highly desirable. The analysis of gene expression profile and microRNA in CCs could bring valuable information about deregulated pathways involved in infertility related to endometriosis, whose understanding will facilitate the investigation of its role in the gestational prognosis and design of the studies for therapeutic intervention. Objectives: Determine the set of mRNAs expressed (transcribed profile) in CCs of infertile women with and without endometriosis (stages I / II and stages III / IV, with and without endometriosis) undergoing controlled ovarian stimulation for ICSI, by next-generation sequencing; and compare the profile of transcripts between the groups proposed. After the identification of differentially expressed genes, we will identify which pathways are involved in these altered genes and correlate with disease and infertility, performing validation of the most promising genes by RT-PCR. Methodology: In the study we will analyze the CCs of 4 of patients: 1 group control, 1 group with endometriosis I / II, 1 group with endometriosis III / IV (with endometrioma) and 1 group with endometriosis III / IV (without endometrioma). All patients will be subjected to the EOC to perform ICSI. Immediately after oocyte retrieval, the CCs are isolated and stored for the next-generation sequencing (RNA-Seq), moreover, analyzes of the expression of the selected target genes by real-time PCR will be performed. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA LUZ, CAROLINE MANTOVANI; DA BROI, MICHELE GOMES; KOOPMAN, LARISSA DE OLIVEIRA; PLACA, JESSICA RODRIGUES; DA SILVA-JR, WILSON ARAUJO; FERRIANI, RUI ALBERTO; MEOLA, JULIANA; NAVARRO, PAULA ANDREA. Transcriptomic analysis of cumulus cells shows altered pathways in patients with minimal and mild endometriosis. SCIENTIFIC REPORTS, v. 12, n. 1, p. 9-pg., 2022-04-06. (14/05878-7)

DA LUZ, CAROLINE M.; DA BROI, MICHELE G.; PLACA, JESSICA R.; SILVA JR, WILSON A.; MEOLA, JULIANA; NAVARRO, PAULA A.. Altered transcriptome in cumulus cells of infertile women with advanced endometriosis with and without endometrioma. Reproductive BioMedicine Online, v. 42, n. 5, p. 11-pg., 2021-04-22. (14/02683-0, 14/05878-7)

DA LUZ, C. M.; MEOLA, J.; DA BROI, M.. DIFFERENTIAL TRANSCRIPT PROFILE OF CUMULS CELLS OF INFERTILE WOMEN WITH AND WITHOUT INITIAL ENDOMETRIOSIS. Fertility and Sterility, v. 108, n. 3, p. 2-pg., 2017-09-01. (14/02683-0, 14/05878-7)

DA LUZ, C. M.; MEOLA, J.; DA BROI, M.; SILVA, L. F.; PLACA, J. R.; SILVA, W. A., JR.; FERRIANI, R. A.; NAVARRO, P. A.. DIFFERENTIAL TRANSCRIPT PROFILE OF CUMULUS CELLS OF INFERTILE WOMEN WITH AND WITHOUT INITIAL ENDOMETRIOSIS WHO UNDERWENT OVARIAN STIMULATION. Fertility and Sterility, v. 108, n. 3, p. 2-pg., 2017-09-01. (14/02683-0, 14/05878-7)