Multi-User Equipment approved in grant 2011/23236-4: automatic sequencing of proteins and peptides, PPSQ-33A model and accessories

• Grant number: 15/02506-4
• Support Opportunities: Multi-user Equipment Program
• Start date: December 01, 2015
• End date: November 30, 2022
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Suely Vilela
• Grantee: Suely Vilela
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis, AP.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Caracterização e Análises de Amostras - Proteínas/Ácidos nuclêicos - Sequenciadores
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The automatic sequencer of proteins/peptides is an equipment whose function is to identify the amino acids which comprise the primary structure of proteins and peptides. This identification occurs through the N-terminal region of the protein by Edman degradation and identification of PTH-amino acids. It is an essential equipment for laboratories working with identification and biochemical characterization of proteins and peptides, which is the case of the Toxinology laboratory of FCFRP-USP. This is a basic equipment for the survival of the research projects of this laboratory, within the time required by financial supporting agencies and graduate courses. There are no longer conditions to attend the demand of the laboratory without the protein sequencing system and peptides by Edman's Method. All isolated proteins need to be submitted to analysis of the sequencer to be characterized as its amino-terminal sequence, which define if the protein is new or has already been identified and characterized in accordance with the published literature. Moreover, it is from these data that the functional characterization experiments will be targeted. From the N-terminal sequencing, and enzymatic or chemical cleavage of the protein with subsequent sequencing of amino acid residues it is possible to determine the primary sequence of the protein which is a fundamental fact to carry out other experiments such as crystallography, molecular modeling, among others. We need to quickly determine the amino terminal sequence of the proteins/peptides to target the experiments of their biochemical and functional characterizations. The equipment will be available to provide services to perform sequencing of proteins and peptides for graduate students of FCFRP and other Schools of the campus, as well as external researchers. It is rare the laboratory that has the equipment, and therefore will be useful for various laboratories working with proteins and peptides. (AU)