Strategies to improve oocyte competence and in vitro embryo production in Holstein dairy cows

• Grant number: 15/19563-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2016
• End date: July 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Pietro Sampaio Baruselli
• Grantee: Pietro Sampaio Baruselli
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Holstein cows during lactation are frequently associated with reduced reproductive efficiency relative the heifers. Among the potential causes can be cited lower oocyte competence which lead to low conception rates and lower efficiency of production programs in vitro embryos (IVP). Changes in energy metabolism appear to influence oocyte competence of lactating females during lactation. Further, it is common to observe dairy cows that did not become pregnant after consecutive four or more services within a single lactation. Those cows are defined as service repeater (RS) and also feature lower oocyte competence. The reduced oocyte quality both in cows in early lactation cows as the RS appears to be associated with altered metabolic profile characterized by peripheral insulin resistance frames (RPI). Thus, the objective of the present study (Exp. 1 and Exp. 2) is to assess: 1) the effect of IR picture of the IVEP RB cows; 2) the impact of lactation disruption on the metabolic profile of RB cows and IVEP of; 3) the new lactation induction on the metabolic profile and IVEP; 4) influence of the concentration of circulating glucose by the infusion of 500 mL of propylene glycol (PPG) 5 days before follicular aspiration on the metabolic profile and IVEP of dairy females of different categories and lactation stages. The hypothesis states that 1) RB cows submitted to stopping lactation and subsequent lactation induction reverse the IR table and consequently improve the in vitro developmental competence of oocytes (Experiment 1-A and 1-B); 2) PPG infusion, changes the metabolic state and improve oocyte quality and IVEP these categories (Experiment 2a and 2b). (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARUSELLI, P. S.; VIEIRA, L. M.; SA FILHO, M. F.; MINGOTI, R. D.; FERREIRA, R. M.; CHIARATTI, M. R.; OLIVEIRA, L. H.; SALES, J. N.; SARTORI, R.. Associations of insulin resistance later in lactation on fertility of dairy cows. Theriogenology, v. 86, n. 1, p. 7-pg., 2016-07-01. (15/19563-0)

PIETRO SAMPAIO BARUSELLI; LAÍS ÂNGELO DE ABREU; VANESSA ROMÁRIO DE PAULA; BRUNO CARVALHO; EMANUELLE ALMEIDA GRICIO; FERNANDO KENJI MORI; LÍGIA MATTOS REBEIS; SOFÍA ALBERTINI; ALEXANDRE HENRILY DE SOUZA; MICHAEL DOCCHIO. Applying assisted reproductive technology and reproductive management to reduce CO2-equivalent emission in dairy and beef cattle: a review. Animal Reproduction, v. 20, n. 2, 2023-09-08. (19/14679-1, 15/19563-0)

BARUSELLI, P. S.; VIEIRA, L. M.; SA FILHO, M. F.; MINGOTI, R. D.; FERREIRA, R. M.; CHIARATTI, M. R.; OLIVEIRA, L. H.; SALES, J. N.; SARTORI, R.. Associations of insulin resistance later in lactation on fertility of dairy cows. Theriogenology, v. 86, n. 1, SI, p. 263-269, JUL 1 2016. (15/19563-0)

BARUSELLI, PIETRO S.; RODRIGUES, CARLOS A.; FERREIRA, ROBERTA M.; SALES, JOSE NELIO S.; ELLIFF, FLAVIA M.; SILVA, LAISA G.; VIZIACK, MARIANA P.; FACTOR, LUANA; D'OCCHIO, MICHAEL J.. mpact of oocyte donor age and breed on in vitro embryo production in cattle, and relationship of dairy and beef embryo recipients on pregnancy and the subsequent performance of offspring: A revie. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 34, n. 2, SI, OCT 2021. (19/14679-1, 15/19563-0)

PIETRO SAMPAIO BARUSELLI; LAÍS ÂNGELO DE ABREU; BRUNA LIMA CHECHIN CATUSSI; ANA CAROLINA DOS SANTOS OLIVEIRA; LÍGIA MATTOS REBEIS; EMANUELE ALMEIDA GRICIO; SOFÍA ALBERTINI; JOSÉ NÉLIO SOUSA SALES; CARLOS ALBERTO RODRIGUES. Use of new recombinant proteins for ovarian stimulation in ruminants. Animal Reproduction, v. 20, n. 2, 2023-09-04. (19/14679-1, 15/19563-0)