Strategies to improve the in vitro embryo production (IVEP) of Holstein (Bos taurus), Gyr (Bos indicus) and Nellore (Bos indicus) young donors

• Grant number: 19/14679-1
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2020
• End date: April 30, 2023
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Pietro Sampaio Baruselli
• Grantee: Pietro Sampaio Baruselli
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Flávia Morag Elliff ; Laísa Garcia da Silva

Abstract

The use of young animals for reproduction is of great interest for the beef and dairy production chains, especially regarding the use of in vitro embryo production (IVEP), because it contributes to the maximization of the herd's potential, allowing the reduction of generation intervals and increase of genetic gain due to rapid dissemination of high value genetic material. Among the factors that may compromise the results of this technology, the donor's age has great importance. Many studies have verified that prepubertal heifers present reduced IVEP efficiency (Presicce et al., 1997; Guerreiro, 2015; Baruselli et al., 2016; Warzych et al., 2017; Bayeux, 2015), which compromises the use of this animal category for IVEP. Therefore, it is of ought most importance to perform studies that search for techniques that may validate and improve the efficiency of IVEP in this animal category. Thus, the objective of Experiment 1 is: 1) evaluate the IVEP of Holstein (Bos taurus) calves (3-8 months) treated with FSH, rBST and the association of FSH + rBST and; 2) compare blood levels of AMH, glucose, insulin and IGF-1 of the animals from each experimental group. The objective of Experiment 2 is: 1) evaluate the IVEP of Gyr (Bos indicus) calves (3-8 months), pubertal heifers (12-22 months) and cows treated or not with FSH and; 2) compare blood levels of AMH, glucose, insulin and IGF-1 of the animals from each experimental group. The objective of Experiment 3 is: 1) evaluate the IVEP of 12 and 24 months old Nellore (Bos indicus) heifers treated or not with FSH and; 2) compare blood levels of AMH, glucose, insulin and IGF-1 of the animals from each experimental group. The hypothesis are 1) Holstein calves treated with FSH and/or rBST have greater follicular diameter at OPU, increase of blood level IGF1 and greater oocyte competence, which improves IVEP when compared to animals that do not receive treatment; 2) Gyr females treated with FSH have greater follicular diameter at OPU and oocyte competence, resulting on improved IVEP when compared to animals that do not receive treatment and; 3) Nellore heifers treated with FSH have greater follicular diameter at OPU and oocyte competence, resulting on improved IVEP when compared to animals that do not receive treatment. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIETRO SAMPAIO BARUSELLI; LAÍS ÂNGELO DE ABREU; VANESSA ROMÁRIO DE PAULA; BRUNO CARVALHO; EMANUELLE ALMEIDA GRICIO; FERNANDO KENJI MORI; LÍGIA MATTOS REBEIS; SOFÍA ALBERTINI; ALEXANDRE HENRILY DE SOUZA; MICHAEL DOCCHIO. Applying assisted reproductive technology and reproductive management to reduce CO2-equivalent emission in dairy and beef cattle: a review. Animal Reproduction, v. 20, n. 2, 2023-09-08. (19/14679-1, 15/19563-0)

BARUSELLI, PIETRO S.; RODRIGUES, CARLOS A.; FERREIRA, ROBERTA M.; SALES, JOSE NELIO S.; ELLIFF, FLAVIA M.; SILVA, LAISA G.; VIZIACK, MARIANA P.; FACTOR, LUANA; D'OCCHIO, MICHAEL J.. mpact of oocyte donor age and breed on in vitro embryo production in cattle, and relationship of dairy and beef embryo recipients on pregnancy and the subsequent performance of offspring: A revie. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 34, n. 2, SI, OCT 2021. (19/14679-1, 15/19563-0)

PIETRO SAMPAIO BARUSELLI; LAÍS ÂNGELO DE ABREU; BRUNA LIMA CHECHIN CATUSSI; ANA CAROLINA DOS SANTOS OLIVEIRA; LÍGIA MATTOS REBEIS; EMANUELE ALMEIDA GRICIO; SOFÍA ALBERTINI; JOSÉ NÉLIO SOUSA SALES; CARLOS ALBERTO RODRIGUES. Use of new recombinant proteins for ovarian stimulation in ruminants. Animal Reproduction, v. 20, n. 2, 2023-09-04. (19/14679-1, 15/19563-0)